Objective: To examine the proportion of atypical squamous cells of indeterminate significance (ASC-US) in postmenopausal women. Examine the correlation of ASC-US cytological findings and pathohistological diagnosis. Subjects and methods: The study included postmenopausal examinees, for which in the period 01/01/2017 - 30/06/2019 during the primary screening for cervical cancer at the Clinical Institute of Clinical Cytology KBC Osijek, cytological examination of VCE smears and cytological diagnosis of ASC-US was performed, under the condition that at that time interval the examinees had pathohistological findings not older than 6 months after the cytological diagnosis. Results: The proportion of ASC-US cytological findings in the category of atypical squamous cells (ASC) in postmenopausal women is 71.1%. It is significantly lower in postmenopausal women than in women of reproductive age. Mixed flora is the most common finding of microorganisms and was observed in 81.4% of examinees. There are no significant differences in the age of the examinees with respect to other microorganisms present. An inadequate cytohormonal status and reactive changes in cells associated with inflammation were observed in a smaller number of examinees (23.7% and 16.9%, respectively). There is no significant difference in the age and number of births of the examinees with regard to the pathohistological finding. The positive predictive value of the ASC-US cytological finding is 36.8%. Conclusions: The cytological finding for ASC-US is characterized by limited morphological interpretation resulting in an indeterminate diagnosis. Adherence to strict cytological criteria for ASC-US, along with recognizing the interpretive traps common to this age group, would reduce its overuse.