Objectives: The aim of this study was to determine the effects of consuming Quattro eggs on macula lutea, especially lutein, as one of the functional elements they are enriched with, by using an optical coherence tomography and also on biochemical and hematological blood parameters in young healthy subjects. Study design: Randomized, double-blind and placebo-controlled trial. Participants and methods: This study included 11 healthy young subjects of both sexes who were randomly assigned to the experimental groups. A Quattro group and a control group were formed. The Quattro group consumed nutritionally enriched hen eggs during the three-week protocol, while the control group included standard hen eggs in their diet. At the beginning and end of a three-week protocol, the subjects underwent ophthalmological examinations of the posterior eye segment by using the OCT method. Furthermore, anthropometric measurements were examined. Venous blood was sampled to determine biochemical and hematological blood parameters. Results: The three-week protocol of consuming nutritionally enriched hen eggs and standard hen eggs significantly influenced some of the examined variables. In the Quattro group changes were observed in the average retinal thickness and in the average retinal volume of anatomical macular regions. No significant changes were found in the retinal thickness of the control group, but the volume of one macular region was reduced. Urea was increased and GGT was significantly reduced in the Quattro group. On the other hand, statistically significant changes in anthropometric variables were observed, SBP and AST were decreased and HR was increased in the control group. Conclusion: Functional ingredients that enriched the chicken eggs have a protective effect on the thickness and volume of the retina. For further research, it is necessary to extend the duration of the protocol, expand the groups, and include subjects with pathological changes of the retina.