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undergraduate thesis
Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma
2021. urn:nbn:hr:152:375390
Sivonjić, Ivona
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Sivonjić, I. (2021). Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:375390

Sivonjić, Ivona. "Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:375390

Sivonjić, Ivona. "Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:375390

Sivonjić, I. (2021). 'Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:375390

Sivonjić I. Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:375390

I. Sivonjić, "Zastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:375390

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Metadata
TitleZastupljenost autofagije u neurosferama dobivenih iz stanica glioblastoma
Title (english)Autophagy abundance in neurospheres derived from glioblastoma cells
AuthorIvona Sivonjić
MentorMarijana Leventić (mentor)
Committee memberBarbara Viljetić (predsjednik povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
Committee memberMarijana Leventić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Cilj istraživanja: Odrediti zastupljenost autofagije u neurosferama dobivenih iz glioblastoma te utvrditi ima li ulogu vremenski period uzgoja neurosfera na pojavnost autofagije. Nacrt studije: Rad je koncipiran kao in vitro studija (kontrolirani pokus). Materijal i metode: Stanična linija D54 nakon uzgoja i kultivacije u inkubatoru nasađena je na mikrotitarsku pločicu sa tzv. V dnom kako bi se omogućilo formiranje sfera. Sferoidi su tako uzgajani kroz 5, 10 i 15 dana. Po isteku vremena stanice su tretirane tripsinom kako bi se razdvojile na pojedinačne stanice koje će se detektirati protočnim citometrom. Za detekciju autofagije obojane su acridin orange fluorescentnom bojom. Izmjerena fluorescencija proporcionalna je razini autofagije u stanicama. Uzorci su analizirani u duplikatu na BD FACScanto II protočnom citometru te su rezultati obrađeni programom FlowJo. Rezultati: Duljim uzgojem sferoida razina autofagije opada. Dokazana je statistički značajna razlika međusobnim uspoređivanjem sferoida formiranih tijekom 5, 10 i 15 dana. Zaključak: Regulatori autofagije su raspodjela hranjivih tvari i kisika koja u 3D staničnoj kulturi više sliči onoj u ljudskom organizmu nego što je slučaj u 2D staničnoj kulturi. Razina autofagije smanjuje se od sferoida starih 5 dana prema sferoidima starim 15 dana jer rastom sferoida raste i zona mrtvih stanica.
Abstract (english)
Objectives: The aim of this study is to determine the rate of autophagy in neurospheres derived from glioblastoma cells and to establish whether the time period of cultivation of spheres affects the occurrence of autophagy. Study design: The study is designed as a controlled experiment. Materials and Methods: After cultivation, the D54 cells were seeded on a V bottom mictotiter plates to allow the formation of spheres. Spheroid were grown for 5, 10 and 15 days. The cells were treated with trypsin to separate the cells which are later detected by a flow cytometer. For autophagy detection, cells were stained with a fluorescent dye, acridine orange. The measured fluorescence is proportional to the level of autophagy. Samples were analyzed in duplicate on a BD FACScanto II flow cytometer and the results were processed by the FlowJo program. Results: With prolonged spheroid cultivation, the level of autophagy decreases. A statistically significant difference was proven by comparing spheroids formed during 5, 10 and 15 days. Conclusion: Distribution of nutrients and oxygen regulate autophagy and are more similar to the one in the human body in 3D cell culture than in the 2D cell culture. The level of autophagy decreases from 5-day-old spheroids to 15-day-old spheroids because as spheroids grow, so does the zone of dead cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:375390
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-01-02 08:56:13