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master's thesis
Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece
2021. urn:nbn:hr:152:181988
Vučemilović-Jurić, Valentina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Vučemilović Jurić, V. (2021). Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:181988

Vučemilović Jurić, Valentina. "Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:181988

Vučemilović Jurić, Valentina. "Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:181988

Vučemilović Jurić, V. (2021). 'Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 March 2024, https://urn.nsk.hr/urn:nbn:hr:152:181988

Vučemilović Jurić V. Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 March 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:181988

V. Vučemilović Jurić, "Povezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:181988

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Metadata
TitlePovezanost polimorfizma rs1045411 HMGB1 gena s pojavnošću IgA vaskulitisa kod djece
Title (english)Relationship of rs1045411 HMGB1 gene polymorphism and incidence of IgA vasculitis in children
AuthorValentina Vučemilović-Jurić
MentorJasenka Wagner Kostadinović (mentor)
Committee memberBarbara Viljetić (predsjednik povjerenstva)
Committee memberSilvija Pušeljić (član povjerenstva)
Committee memberJasenka Wagner Kostadinović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Ciljevi istraživanja: Cilj ovog istraživanja bio je utvrditi postoji li povezanost polimorfizma gena za HMGB-1 (rs1045411) i pojave IgA vaskulitisa metodom lančane reakcije polimeraze u stvarnom vremenu. Ustroj studije: Studija slučajeva i kontrola. Ispitanici i metode: Ispitanici u ovom istraživanju su djeca, njih 176; 100 zdravih kontrola i 76 oboljelih s dijagnozom IgAV. Ispitanici s IgAV upućeni su iz Klinike za pedijatriju Kliničkog bolničkog centra Zagreb i Kliničkog bolničkog centra Osijek. Krv svih ispitanika uzorkovana je minimalno invazivno, jednokratnom venepunkcijom, za potrebe genotipizacije. Svim ispitanicima napravljena je genotipizacija real-time PCR metodom na uređaju 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) koristeći TaqMan SNP hibridizacijske probe. Rezultati: Prilikom usporedbe frekvencije genotipa i alela HMGB genskog polimorfizma rs1045411 kod pacijenata oboljelih od IgAV s frekvencijom genotipa i alela u kontrolnoj skupini, nije pronađena statistički značajna razlika (p > 0,05). Također, nije uočena povezanost između proučavanih genotipa i razvoja IgAVN kao komplikacije IgAV (p > 0,05). Zaključak: Ovim istraživanjem utvrđeno je da ne postoji povezanost polimorfizma gena za HMGB1 (rs1045411) i pojave IgAV, kao ni povezanost s povećanim rizikom od razvoja IgAVN u IgAV.
Abstract (english)
Objectives: The aim of this study was to investigate the possible role of single nucleotide polymorphism rs1045411 of the HMGB1 gene in occurrence of IgAV vasculitis using real-time PCR genotyping. Study design: Case-control study Participants and methods: The subjects in this study were children, 176 of them; 100 healthy controls and 76 patients diagnosed with IgAV. The subjects with IgAV were referred from the Clinic for Pediatrics of the Clinical Hospital Centre Zagreb and the Clinical Hospital Centre Osijek. The blood of all subjects was sampled minimally invasively, with a single venipuncture, for the purposes of genotyping. All subjects were genotyped using real-time PCR on a 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using TaqMan SNP genotyping assays. Results: When comparing the frequency of genotypes and alleles of the HMGB rs1045411 gene polymorphism in IgAV patients with the frequency of genotypes and alleles in the control group, no statistically significant difference was found (p > 0.05). Also, no association was observed between the studied genotypes and the development of IgAVN as a complication of IgAV (p > 0.05). Conclusion: This study has shown that there was no association between the polymorphism of the HMGB1 gene (SNP rs1045411) and the IgAV occurrence, nor an association with an increased risk of developing IgAVN in IgAV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:181988
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-01-10 10:42:25