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undergraduate thesis
Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora
2022. urn:nbn:hr:152:402653
Kovač, Lora
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Kovač, L. (2022). Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:402653

Kovač, Lora. "Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:402653

Kovač, Lora. "Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:402653

Kovač, L. (2022). 'Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:402653

Kovač L. Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:402653

L. Kovač, "Utjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:402653

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Metadata
TitleUtjecaj kisika na izražaj gena adenozinskih A1 i A2a receptora u krvnim žilama Sprague-Dawley štakora
Title (english)Influence of oxygen on adenosine A1 and A2a receptor gene expression in blood vessels of Sprague-Dawley rats
AuthorLora Kovač
MentorZrinka Mihaljević (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAleksandar Kibel (član povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Cilj istraživanja: Utvrditi utjecaj akutnog i intermitentnog izlaganja hiperbaričnoj oksigenaciji (HBO2) na izražaj gena adenozinskih receptora (A1 i A2a) u krvnim žilama mozga SpragueDawley štakora. Nacrt studije: Eksperimentalna studija na izoliranim organima pokusnih životinja (SpragueDawley štakori). Materijal i metode: Zdravi Sprague-Dawley štakori oba spola, starosti 8 - 10 tjedana, podijeljeni su u tri skupine: KONTROLA (kontrolna skupina, nije izložena HBO2), A-HBO2 (akutno izložena skupina, jednom izložena HBO2 u trajanju od 2 sata pod tlakom od 2 bara i potom žrtvovana) i 4D-HBO2 (intermitentno izložena skupina, izložena HBO2 po 2 sata dnevno kroz 4 dana, uzorkovanje je obavljeno peti dan). Uzeti uzorci površinskih krvnih žila mozga korišteni su za RTqPCR. Određen je relativni izražaj A1 i A2a gena uz korištenje HPRT1 kao housekeeping gena. Rezultati: Akutno izlaganje hiperbaričnoj oksigenaciji nije značajno utjecalo na izražaj A1 ili A2a gena. Uočen je značajan porast izražaja A1 i A2a gena u intermitentno izloženoj skupini u odnosu na kontrolnu skupinu. Zaključak: Akutno izlaganje HBO2 ne pokazuje značajan učinak na izražaj adenozinskih receptora, dok intermitentno izlaganje HBO2 djeluje kao pseudohipoksija, koja potiče povećanje izražaja A1 i A2a gena.
Abstract (english)
Objectives: This study aimed to determine the influence of acute and intermittent hyperbaric oxygenation (HBO2) on the expression of adenosine receptor genes (A1 and A2a) in brain blood vessels of Sprague-Dawley rats. Study design: Experimental animal study using isolated organs of Sprague-Dawley rats. Material and methods: Healthy, male and female Sprague-Dawley rats (aged 8 - 10 weeks) were randomly divided into three groups (N=18, 6 rats per group): CONTROL group (was not exposed to HBO2), acute HBO2 group (A-HBO2, exposed to the HBO2 protocol once and sacrificed immediately after) and intermittent HBO2 group (4D-HBO2, exposed to the HBO2 protocol during four consecutive days and sacrificed on the fifth day). The HBO2 protocol consisted of two hours of exposure to 100% oxygen under 2 bars of pressure. Afterwards, samples of surface brain blood vessels were obtained and used to extract RNA. Using quantitative RT-PCR, relative gene expression of A1 and A2a receptor genes was measured using HPRT1 as the housekeeping gene. Results: Acute exposure to HBO2 did not significantly impact the gene expression of A1 or A2a, while there was a significant increase in both A1 and A2a gene expression in the intemittent group compared to the CONTROL group. Conclusion: Acute exposure to HBO2 does not significantly alter adenosine receptor gene expression, whereas intermittent exposure acts as pseudohypoxia, leading to an increase in A1 and A2a gene expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:402653
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2024-03-12 12:22:42