OBJECTIVE: To examine how familiar high school graduates are with medical laboratory diagnostics as a profession and to examine whether graduates are interested in enrolling in studies in the field of medical laboratory diagnostics. STUDY DESIGN: The research was organized as a cross-sectional study. PARTICIPANS AND METHODS: The study was conducted at the Isidor Kršnjavog High School in Našice. Students of four-year programs participated in the examination. The criterion for inclusion in the research is the age of 18 years. 74 students participated in the research. A survey questionnaire was used as a research instrument, and the personal data of the students were not used in the research. RESULTS: 74 students of Izidor Kršnjavo High School in Našice participated. Graduates' familiarity with the study of medical laboratory diagnostics is very low, which may be the result of insufficient information about that study. Although the majority of respondents do not have enough information, about 50% of respondents agree with the statement that to work in the laboratory it is necessary to have developed routine skills and that constant improvement of knowledge and skills is necessary. Linking the lack of knowledge and lack of information of graduates, a small number of them are interested in enrolling in the study of medical laboratory diagnostics. Comparing the results of graduates that the study of medical diagnostics is difficult and the importance of safety and health protection related to medical diagnostics, the majority of respondents strongly disagree that they are ready to invest time and effort in the study of medical-laboratory diagnostics. CONCLUSION: An insufficient number of graduates are instructed in the study of medicallaboratory diagnostics and have an insufficient amount of information about this study, so educational institutions should advertise the profession of medical diagnostics more and provide more information in order to increase interest in this study, given that a small number of students have the desire enroll in the study of medical-laboratory diagnostics.