Aim: To examine the difference in the opinion regarding nursing profession among nursing students of the first and third year of the University Undergraduate Study of Nursing and students of the second year of the University Graduate Study of Nursing at the Medical Faculty of Osijek, as well as the influence of education on students' opinions about nursing as a profession. Examinees and methods: 123 students participated in this research. Research was carried out at the Medical Faculty of Osijek. A survey was created and used as an instrument for the requirements of this research. Descriptive statistic analysis was used. Results: When it comes to students' year of study, no statistically significant difference was found in any of the claims about the perception of nursing as a profession. However, regarding social values of nursing, statistically significant differences were found in the medians of the statement “Nurses are respected in society”, with undergraduate students giving the most positive answers, and students of the second year of graduate study providing the most negative ones (p = 0.028). A similar contrast is found in the statement: “There is an adequate representation of work done by nurses and medical technicians in the media” (p = 0.031). Statistically significant differences in the existence of the awareness of nursing as a profession can be seen in the belief that “Nurses/medical technicians earn a lot of money”, which is evident in the shift of the interquartile range value from 2-3 for the two years of undergraduate study to 1-2 for the second year of graduate study (p = 0.027). A similar difference is observable in the statement that “A nurse/medical technician has the same authority as the other members of the team”, a statement that the second-year graduate students agree with the least (p <0.001). Conclusion: There is a difference in students' opinion depending on their year of study, whereas students' opinion regarding some of the statements is changing with the process of studying.