OBJECTIVE: The aim of this study was to investigate the opinions and knowledge of nursing students about the prevention of cervical cancer, to determine whether there are differences in opinion and knowledge between the first year and final year nursing students. ORGANIZATION OF THE STUDY: The study is structured as a cross-sectional study. SUBJECTS AND METHODS: The subjects were first year and final year students at the undergraduate nursing study. The method of data collection is a survey using a questionnaire titled "Sexual Behavior, Knowledge and Attitudes About the Prevention of Cervical Cancer", written by Ljiljana Antic (with permission of the author). RESULTS: A total of 77 female subjects/first year and third year students at the undergraduate nursing study participated. Half of the students visit a gynecologist once a year, only one quarter had never visited a gynecologist, and nearly 38% do a Pap smear once a year. The unsatisfactory result is that more than half of the subjects believe that condoms protect against HPV infection. Sixty percent of students believe that the vaccine is effective and they would recommend it. Seventy percent of female third year students received the most information about prevention of cervical cancer in class, while 40% of female first year students received information via the internet. CONCLUSION: A large percentage of female students of both generations are visiting their gynecologist regularly. Significantly more of the third year students answered questions about HPV infection and cervical cancer prevention accurately. The conclusion of this study is that it is necessary to conduct education and encourage women of all age groups to regularly visit their gynecologist because that is the key to cervical cancer prevention.