The aim of the research: the objective of this research was to test the knowledge and opinions of students and parents, to disconcert the differences in gaining information, about a desirable future way of informing, and whether epidemiological factors influence on the general awareness about the Down syndrome. Plan of the study: the cross-sectional study was conducted at the Medical College and the Department of Pediatrics in KBC Osijek in May and June 2016. Participants and methods: the research was conducted on the sample of 46 students and 60 parents, 15 male and 91 female participants in the ages between 20 to 57. The testing was done anonymously and with informed consent. An anonymous questionnaire was used, and it was comprised of 22 questions, 4 personal data questions, 12 questions on the knowledge about the Down syndrome and 6 questions about opinions and education about the Down syndrome. All the answers had multiple choices except the subjects’ age. Results: based on the results we have concluded that both parents and students have good knowledge and opinions about the children with the Down syndrome. Through additional testing, we have concluded that there is a significant difference in knowledge and opinions of both students and parent if we take into the consideration their gender, age, and level of education. Conclusion: based on the results of this research we can say that both students and parents have some knowledge about the children with the Down syndrome. To improve their knowledge, it is necessary to organize special educational programs in schools and to attribute more importance to the children with the syndrome through the media. Both parents and students have shown very positive attitudes toward the people with the Down syndrome.