Objective: The aim of this study was to analyze and compare the risk factors for chronic venous insufficiency in the population of medical professionals working in County Hospital Čakovec. An additional aim was to investigate the relationship between duration of shift work and the presence of risk factors for the development of chronic venous insufficiency. Methods: The study was conducted by anonymous questionnaires obtained from volunteering participants. Previously published and validated, publicly available questionnaire Phleboscore from pharmaceutical company Servier was used. Phleboscore questionnaire has 11 questions with socio-demographic data on gender and age indicating risk of chronic venous insufficiency. Eight additional questions made by author were added to the questionnaire in order to investigatespecific risk factors for chronic venous insufficiency related to working in hospital environment. Results: Results of this study show that with regard to the limit score of 12 (determined according to the original publication), in 110 (55%) of respondents there was no risk of CVI, while 90 (45%) were at increased risk of CVI. Participants who are working in shifts for more than 10 years had a significantly higher risk for CVI than those who work in shifts for a shorter duration of time (Kruskal Wallis test, P <0.001). In addition, subjects who had positive medical history for deep vein thrombosis were atsignificantly higher risk for CVI (Mann Whitney U test, P = 0.003) compared to those with negative medical history. Conclusion: The survey showed that 45% of physicians and nurses working in County Hospital Čakovec were at increased risk for CVI. Most of them work in shifts which proved to be a risk factor that contributes to the development of symptoms of CVI especially if they work for more than 10 years.