Research objective: The main aim was to examine the attitudes of pregnant women and their partners towards vaccination based on sex, age, professional qualification, place of residence and the difference in attitudes between pregnant women and their partners. Subjects and methods: Subjects were pregnant women (N = 55) and their partners (N = 55). As a research instrument, a survey sheet with demographic data and questionnaires of PACV and SAGE group were used. The study was conducted from September to November 2016 during the pregnancy course at the Osijek Health Center. Results: Out of 110 respondents on the PACV scale 74 (67.2%) of them have a score higher than 25. Out of a total number of 29 (26.6%) of the respondents who live in the countryside, a significantly larger number of them have a score higher than 25 (Fisher's exact test, P = 0.005). In the SAGE group questionnaire, the central value of the whole scale of attitudes is 35 (interquartile range from 22 to 50 points) ranging from 3 to 67 points. Residents who live in the city have significantly lower values of the whole scale, median 30 (interquartile rapson from 17 to 43), compared to those from the countryside (Mann Whitney U test, P = 0.001). There was no significant difference noticed based on gender, age and professional qualification in both questionnaires. Conclusion: Pregnant women and their partners have an indecisive attitude towards vaccination. Respondents from the countryside are more indecisive towards vaccination than the respondents from the city. Pregnant women and their partners did not differ significantly in their attitudes about vaccination except with regard to side effects where the partners were more concerned.