Aims: to examine the situations and events in the workplace which are recognized by health workers as contributing to the development of stress; to identify the differences in workplace situations and events which contribute to the development of stress regarding the age, gender, qualification, occupation and length of service. Study design: A cross-sectional study. Methods and participants: the research was conducted in August, 2017. The study included 132 respondents, 50 doctors and 82 nurses from the Clinical hospital centre Osijek. Stressors, which were used at the workplace of hospital health workers, for the purpose of this research, consisted of 17 different situations which are considered to be the most common ones which cause stress. Results: most of the respondents are female. The average age of the respondents was 41.27 years. The results of this study show that 98% of doctors have high qualifications compared to nurses (12%). Doctors at the Clinical hospital centre Osijek believe that a major source of stress for them is work overload, working in shifts, paperwork and an insufficient number of staff, while nurses think that a major source of stress for them are fewer promotion opportunities. Conclusion: excessive work, paperwork, insufficient number of employees, the threat of a law suit, conflicts with patients and limited time for the examination of patients are the most common stress sources for nurses and physicians. There is no significant difference in situations which contribute to the development of gender-related stress. There are differences in situations and workplace events which contribute to the development of stress in terms of age, vocational qualifications, occupation, and length of service