Objectives: To determine the number of patients who were isolated due to hospital infections/colonisations in the Pula General Hospital in the period from January to December 2016, the departments which are mostly at risk of hospital infections, the age, sex and the most common hospital infection agents in respect to positive microbiological isolates. Study Design: Retrospective research Material and methods: The research included 224 patients which suffered from a hospital infection in the Pula General Hospital. The research was conducted in the period from 1 January 2016 to 31 December 2016. Patients were divided according to age, sex, hospital infection agents in respect to positive microbiological isolates, then the place where the agents were isolated and departments of occurrence. Results: Of the total number of hospitalized patients, or 17,952 of them (10,196 women and 7,756 men), the number of isolated patients where the suspicion of a hospital infection was risen amounts to 224 (1.24 %), 104 of them being women and 120 men. The isolation procedure included 196 patients, while the isolation procedure for the remaining 28 patients was not indicated. The average respondents’ age was 70 (ranging from 00 to 98 years of age). Of all the isolated patients, the largest number were male patients and patients from 0 to 10 years old. The most common hospital infection/colonisation agent in that period was the bacteria Klebisiella pneumoniae MDR. The largest number of positive isolates was found in urine. The department with the largest number of isolated patients in that period was the Department of Infectious Diseases. Conclusion: The research included 224 patients which suffered from a hospital infection in the Pula General Hospital. The research was conducted in the period from 1 January 2016 to 31 December 2016. Patients were divided according to age, sex, hospital infection agents in respect to positive microbiological isolates, then the place where the agents were isolated and departments of occurrence.