Objective: The objective of this research is to examine hormonal levels of participants and their influence on male infertility, and to examine if there is a correlation between hormonal levels and levels of parameters of spermiogram. Study design: This study is designed as a cross-sectional study. Participants and methods: The study included patients who have been treated under diagnose of male infertility in the Department of Urology in Clinical Hospital Osijek in 2017. The data were collected in April 2018 from database of Department of Urology. Hormones examined in research are follicle-stimulating hormone, luteinizing hormone, prolactin, thyroid-stimulating hormone, testosterone, estradiol, triiodothyronine and thyroxine. Hormone levels were determined from blood samples by CMIA method. Variables of spermiogram in this research are volume, pH, concentration, number, vitality, motility and morphology of sperms and levels of zinc, fructose and citrates. Spermiogram was processed from ejaculate samples. Participants were classified in relation to reference values and then set in interrelations. Results: The study included 49 infertile males. 15 (31 %) of them were suspect for deviation of hormone levels. The median age of participants was 33 (interquartile range from 30 to 37 years) in range from 21 to 44 years. Values of follicle-stimulating hormone, thyroxine, thyroid-stimulating hormone and prolactin were in all cases in reference range. One participant had decreased level of testosterone, one decreased level of luteinizing hormone, two had decreased level of thyroid-stimulating hormone and two had increased level of estradiol. There was a negative correlation between luteinizing hormone and concentration, number and morphology of sperms, and there was also a negative correlation between testosterone and concentration, number, vitality and morphology of sperms. Conclusion: Deviations from reference values in hormonal levels are a factor in male infertility. The most significant are luteinizing hormone and testosterone with their negative correlation to certain parameters of spermiogram.