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undergraduate thesis
CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA
2018. urn:nbn:hr:152:152228
Živković, Zorislava
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Živković, Z. (2018). CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:152228

Živković, Zorislava. "CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:152228

Živković, Zorislava. "CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:152228

Živković, Z. (2018). 'CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:152228

Živković Z. CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:152228

Z. Živković, "CITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:152228

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Metadata
TitleCITOTOKSIČNI UČINCI NOVIH DERIVATA 7 – KLORKINOLINA
Title (english)CITOTOXIC EFFECTS OF NEW DERIVATIVES 7 - CHLORININOLINE
AuthorZorislava Živković
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberTeuta Opačak-Bernardi (predsjednik povjerenstva)
Committee memberStana Tokić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-07-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Uvod: Zbog njihove biološke i farmakološke aktivnosti, kinolini i njihovi derivati, klorkinolini, postali su jedni od najpopularnijih N-heteroaromatičnih spojeva koji se često koriste kao ključna komponenta u proizvodnji lijekova. Derivati kinolina pokazali su se uspješnima kao kemoterapeutici i citotoksični agensi. Učinkovitost im se očituje u inhibiciji proliferacije tumorskih stanica, angiogeneze i remećenju metastaziranja. Ciljevi istraživanja: Cilj rada bio je odrediti citotoksičan učinak derivata 7-klorkinolina na panelu humanih tumorskih stanica i jednoj normalnoj psećoj staničnoj liniji. Materijali i metode: Derivati su pripremljeni u Zavodu za kemiju i biokemiju Veterinarskog fakulteta u Zagreb. Za potrebe in vitro istraživanja derivati 7-klorkinolina otopljeni su u DMSO-u (dimetil sulfoksidu) kao koncentrirane 10⁻2 mol/dm3 otopine. Kultivacija stanica odvijala se u inkubatoru s kontroliranom atmosferom uz 5 % CO₂ i temperaturom od 37 °C u bočicama za uzgoj stanica površine 25 cm2. Za održavanje adherentnih staničnih linija (HeLa, CaCo-2 i MDCK-1) upotrebljava se DMEM medij. RPMI medij koristio se za održavanje suspenzijske K562 stanične linije. Inhibitorni učinak određen je MTT testom. Rezultati: Proliferaciju testiranih stanica u najvećoj mjeri inhibirao je derivat MB-127 pri najvišoj koncentraciji. Derivat MB-103 je najmanji citotoksičan učinak imao na MDCK-1 i CaCo-2 stanične linije. Stanični rast linije K562 pokazao se kao najosjetljiviji na učinke derivata 7-klorkinolina. Zaključak: Derivati klorkinolina smanjuju stanični rast tumorskih stanica u ovisnosti o koncentraciji i tipu stanica. Selektivno djelovanje derivata na normalne stanice nije uočeno.
Abstract (english)
Introduction: Due to their biological and pharmacological activity, quinolines and chloroquinoline derivatives have become some of the most popular N-heteroaromatic compounds frequently used as a key component in drugs production. Quinoline derivatives have proved to be efficient as chemotherapeutic and cytotoxic agents. They inhibit proliferation of tumor cells, angiogenesis and metastatic progression. Objectives: The aim was to determine the cytoxic effect of 7-chloroquinoline derivatives on a panel of human tumor cells and one normal canine cell line. Materials and methods: Derivatives were prepared at the Faculty of Veterinary Medicine in Zagreb, Department of Chemistry and Biochemistry. For in vitro analysis, 7-chloroquinoline derivatives were dissolved in DMSO (dimethyl sulfoxide) as 10-2 mol/dm3 solutions. The cells were grown in 25 cm2 flasks, in a CO2 incubator at 37ºC with 5% CO2. For maintaining the adherent cell lines (HeLa, CaCo-2 i MDCK 1) a DMEM medium was used. K562 cell line was maintained in RPMI medium. The inhibitory effect was determined by the MTT assay. Results: Proliferation of the tested cells was significantly inhibited by the derivative MB-127 at the highest concentration. The derivative MB-103 had the slightest cytotoxic effect against MDCK and CaCo-2 cell lines. The cell growth of K562 line proved to be the most sensitive to the effects of the 7-chloroquinoline. Conclusion: Chloroquinoline derivatives reduce tumor cells growth depending on the concentration and the type of cells. The selective effect of derivatives on normal cells was not observed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:152228
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2018-08-30 08:24:30