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master's thesis
Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom
2018. urn:nbn:hr:152:178537
Katić, Aleksandar
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Katić, A. (2018). Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:178537

Katić, Aleksandar. "Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:178537

Katić, Aleksandar. "Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:178537

Katić, A. (2018). 'Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:178537

Katić A. Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:178537

A. Katić, "Promjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:178537

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TitlePromjene pokazatelja upale u visceralnom masnom tkivu Sprague Dawley štakora hranjenih masnom i slatkom hranom nakon terapije metforminom i liraglutidom
Title (english)Changes in visceral fat tissue inflammatory markers in Sprague Dawley rats on high fat high sugar diet after treatment with metformin and liraglutide
AuthorAleksandar Katić
MentorMarija Heffer (mentor)
Committee memberJasenka Wagner Kostadinović (predsjednik povjerenstva)
Committee memberNikola Bijelić (član povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Glavni je cilj ovog istraživanja odrediti postoji li razlika u veličini masne kapljice i stupnju upale u visceralnom masnom tkivu nakon dijete bogate mastima i ugljikohidratima (HFHSD engl. high fat high sugar diet) te nakon iste dijete, ali uz primjenu monoterapije metformina i liraglutida. Nacrt studije: Istraživanje je provedeno kao studija parova. Materijali i metode: U istraživanje su uključene 64 životinje podijeljene u 8 skupina, 4 skupine mužjaka i 4 skupine ženki. Prva je skupina bila na standardnoj dijeti, druga skupina na HFHSD-u, treća skupina na HFHSD-u i tretirana metforminom te četvrta skupina na HFHSD-u tretirana liraglutidom. Primijenjeno je histološko bojenje hematoksilinom i eozinom te je određena veličina masnih kapljica u skupinama. Stupanj upale mjerio se imunohistokemijskim bojenjem na markere makrofaga. Rezultati su kvantificirani ImageJ programom. Rezultati: Veličina masne kapljica povećana je u drugoj i trećoj skupini mužjaka i ženki štakora, dok je u četvrtoj skupini ženki štakora veličina masne kapljice smanjena. Metformin u mužjaka štakora smanjuje broj sveukupnih makrofaga, dok kod ženki štakora smanjuje broj protuupalnih makrofaga. Liraglutid povećava broj protuupalnih makrofaga kod mužjaka štakora, a kod ženki štakora povećava broj sveukupnih makrofaga. Zaključak: HFHSD dovodi do povećanja masne kapljice i do subkliničke upale u visceralnom masnom tkivu. Antidijabetici metformin i liraglutid djeluju na metabolizam visceralnog masnog tkiva i postoji o spolu ovisno djelovanje. Metformin i liraglutid smanjuju stupanj upale u mužjaka, no takav učinak nemaju kod ženki štakora.
Abstract (english)
Objectives: The main goal of this study is to determine whether there is a difference between the size of lipid vacuole and degree of inflammation in visceral fat tissue after a diet rich in fat and sugar, and the same diet in subjects treated with metformin and liraglutide. Study design: The study was conducted as a matched pair study. Materials and Methods: 64 animals were divided into 8 groups, 4 female and 4 male groups. The first group had the standard diet; the second had the high fat high sugar diet (HFHSD); third and fourth also had HFHSD, but additionally the third group was given metformin, and the fourth group liraglutide. Hematoxylin and eosin histological staining of visceral adipose tissue was used to measure the size of lipid vacuole. The degree of inflammation is estimated imunohistochemically by using CD68 and CD163 markers. The results were quantified using ImageJ software. Results: The size of lipid vacuoles increased in the second group in both male and female rats. Metformin caused a decrease of overall macrophage number in male rats, and decrease in the number of anti-inflammatory macrophages in female rats. Liraglutide increased the number of anti-inflammatory macrophages in male rats, and increased the overall number of macrophages in female rats. Conclusion: HFHSD increases the size of adipocytes and causes a sub-clinical inflammation in the visceral adipose tussie. Anti-diabetics metformin and liraglutide have a sex specific effect on visceral fat; they decrease the inflammation in the tissue of male rats, but don't have the same effect on female rats.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:178537
Study programmeTitle: University Graduate Studies
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-12-05 12:48:01