Introduction: Obesity is caused by excessive food intake and is a risk factor for a number of chronic diseases, including diabetes. Type 2 diabetes is a metabolic disorder with a resistance to insulin. Consequently, there are many changes in the body, among which are iron metabolism disorders in the liver and brain. Using animal models of prediabetes, it is possible to monitor changes in the body and the effect of antidiabetic therapy on these changes. Goals: To evaluate iron content in the liver and substance nigra (SNR) by staining according to Perls method, and in the SNR region by immunohistochemistry on transferrin receptor (TFR). To evaluate the amount of amyloid on brain slices using histological color Congo red. To evaluate the regenerative liver function by using immunohistochemical methods Materials and methods: The study included 32 male Sprague Dawley rats divided into 4 groups: 1 animals on standard diet, 2 animals on high fat and high sucrose diet, 3 animals on enriched diet treated with metformin, 4 animals on enriched diet treated with liraglutide. With histological stainings by the Perls method we detected iron, and by Congo red we detected amyloid. Using immunohistochemical analysis by antibodies for TFR, phosphorylated Tau (pTau), neuronal nitric oxide synthase (nNOS) and proliferating cell nuclear antigen (PCNA), respective markers were detected. Results: The results showed statistically significant differences in case of staining for iron and PCNA antigen, while in the case of pTau, nNOS and Congo red staining there were no statistically significant differences. Antidiabetics metformin and liraglutide failed to demonstrate their therapeutic effects in the conducted study. Conclusion: Prediabetes causes iron metabolism disorders in the liver and brain which lead to tissue damage.