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undergraduate thesis
Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom
2018. urn:nbn:hr:152:248472
Jakšić, Petra
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Jakšić, P. (2018). Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:248472

Jakšić, Petra. "Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:248472

Jakšić, Petra. "Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:248472

Jakšić, P. (2018). 'Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:248472

Jakšić P. Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:248472

P. Jakšić, "Uloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:248472

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Metadata
TitleUloga Tempol-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom
Title (english)Role of Tempol on the flow-induced dilation in Sprague-Dawley rats on Losartan therapy
AuthorPetra Jakšić
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Glavni cillj ovog istraživanja je odrediti učinak TEMPOL-a na protokom potaknutu dilataciju kod Sprague-Dawley štakora na terapiji Losartanom. Materijali i metode: U istraživanje su bili uključeni zdravi muški Sprague-Dawley (SD) štakori podijeljeni u dvije skupine: zdravi netretirani štakori (N = 9) te štakori na terapiji Losartanom (N = 10). Prije dekapitacije štakori su anestezirani ketaminom (75 mg/kg) i midazolamom (0,5 mg/kg). Srednje cerebralna arterija je izolirana i inkubirana pri tlaku Δ80 mmHg. Zatim se mjerio promjer ovisno o tlačnim gradijentima, prvo bazalne vrijednosti, a zatim u prisutnosti TEMPOL-a te TEMPOL-a i L-NAME-a (inhibitor NO sintaze) ovisno o tlačnim gradijentima. Na kraju protokola odredio se maksimalni promjer krvne žile. Rezultati: Rezultati pokazuju da TEMPOL (100 μmol/L) in vitro poboljšava razinu dilatacije u eksperimentalnoj skupini životinja koje su bile na terapiji Losartanom dok u kontrolnoj skupini TEMPOL nije imao učinka na promjenu razine dilatacije središnje moždane arterije. Naknadno dodan in vitro L-NAME (nakon TEMPOL-a) značajno smanjuje razinu protokom potaknute dilatacije, što potvrđuje povećanu razinu oksidativnog stresa stvorenog djelovanjem Losartana. Zaključak: Dokazano je da primjena TEMPOL-a ne dovodi do promjene razine protokom potaknute dilatacije središnje moždane arterije kod zdravih kontrolnih SD štakora dok kod tretiranih životinja TEMPOL značajno oporavlja narušenu protokom potaknutu dilataciju nastalu primjenom Losartana, što dokazuje povećanu razinu oksidativnog stresa nastalog djelovanjem Losartana. Značajna uloga NO-a u mehanizmu protokom potaknute dilatacije potvrđena je dodavanjem inhibitora NO sintetaze nakon inkubacije TEMPOL-om kod tretiranih životinja, nakon čega smo dobili značajno smanjenu protokom potaknutu dilataciju.
Abstract (english)
Objective: The main goal of this study was to determine the role of TEMPOL in the flow-induced dilatation in Sprague-Dawley rats on Losartan therapy. Methods: Healthy male SD rats were divided into two groups: healthy untreated rats (N = 9) and the rats on Losartan therapy (N = 10). Prior to decapitation, the rats were anesthetized with ketamin (75 mg/kg) and midazolam (0,5 mg/kg). The middle cerebral artery was isolated and incubated at a pressure of Δ80 mmHg. After that a basal diameter values were measured depending on the pressure gradients. After the basal diameter values were determined, the blood vessel diameter was measured in the presence of TEMPOL, and TEMPOL and L-NAME (NO synthase inhibitor), depending on the pressure gradients. In the end the maximum blood vessel diameter was measured. Results: The results show that TEMPOL (100 μmol/L) in vitro improved the level of dilatation in experimental group of animals treated with Losartan therapy, but it did not have an effect on the level of the middle cerebral artery dilatation in the control group. Subsequent addition of in vitro L-NAME (after TEMPOL) significantly reduces the level of flow-induced dilatation, which confirms the increased level of oxidative stress caused by Losartan therapy. Conclusion: TEMPOL does not change the level of flow-induced dilatation of the middle cerebral artery in healthy untreated SD rats, while in treated animals TEMPOL significantly recovers the impairment of flow-induced dilatation caused by the use of Losartan, proving the increased oxidative stress caused by Losartan. Significant role of NO in the mechanism of flow-induced dilatation was confirmed by adding NO synthase inhibitors after incubation with TEMPOL in treated animals. After that the flow-induced dilatation was significantly reduced.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:248472
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-01-11 09:19:49