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master's thesis
Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom
2018. urn:nbn:hr:152:428895
Grgurić, Tamara
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Grgurić, T. (2018). Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:428895

Grgurić, Tamara. "Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:428895

Grgurić, Tamara. "Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:428895

Grgurić, T. (2018). 'Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:428895

Grgurić T. Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:428895

T. Grgurić, "Histološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:428895

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Metadata
TitleHistološka analiza utjecaja nedostatka interferonskih receptora alfa i beta na knock-out miševima inficiranim citomegalovirusom
Title (english)Histological analysis of the impact of the lack interferon receptors alpha and beta in the knock- out mice infected with cytomegalovirus
AuthorTamara Grgurić
MentorSvjetlana Marić (mentor)
Committee memberBranko Dmitrović (predsjednik povjerenstva)
Committee memberTatjana Belovari (član povjerenstva)
Committee memberSvjetlana Marić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Cilj istraživanja: Cilj ovog rada bio je na tkivnim uzorcima miševa soja C57/B6 i IFNαβR KO, koji su inficirani citomegalovirusom prvog dana po okotu, ustanoviti prisutnost virusa, odnosno promjene uzrokovanje infekcijom. Nacrt studije: Istraživanje parova Ispitanici i metode: Istraživanje je provedeno na miševima soja C57/B6 i IFNαβR KO. Iz svakog soja korištene su po dvije životinje: jedna kontrolna koja je primila samo medij bez virusa i druga koja je inficirana divljim tipom citomegalovirusa. Životinje su žrtvovane četvrtog dana po okotu te im je od jetre i žlijezda slinovnica pripremljeno 14 inficiranih i 14 kontrolnih preparata za daljnje analize. Preparati su tretirani dvjema metodama, rutinskom hemalaun-eozin metodom i imunohistokemijskom metodom. Kod imunohistokemijske metode korišteno je primarno protutijelo Croma 101, mišje monoklonsko protutijelo specifično za virusni fosfoprotein pp89. Rezultati: Mikroskopskom analizom preparata obojenim hemalaun-eozin metodom u jetri i žlijezdama slinovnicama mogu se uočiti promjene uzrokovane virusom, eksprimirane niskim intenzitetom. Primjenom imunohistokemijskog bojenja preciznije su detektirane promjene uzrokovane virusom, zahvaljujući utvrđivanju mjesta ekspresije virusnog proteina pp89. Zaključak: Promjene uzrokovane citomegalovirusom znatno su izraženije kod miševa soja IFNαβR KO, koji je imunodeficijentan zbog nedostatka interferonskih receptora, u odnosu na soj C57/B6, kod kojeg je na suzbijanje infekcije djelovala aktivnst interferonskih receptora.
Abstract (english)
Objectives: The aim of this study was to detect the presence of cytomegalovirus on the tissue samples from mice of C57/B6 and IFNαβR KO strains, infected with cytomegalovirus on day one after birth, as well as to detect the changes caused by the infection. Study Design: Case-control study Material and methods: The study was performed on mice of C57/B6 and IFNαβR KO strains. Two animals of each strain were used, one as a control, that received only a virus-free medium and another infected with a wild-type cytomegalovirus. The animals were sacrificed on the fourth day after birth, and 14 infected and 14 control tissue samples of liver and salivary glands were prepared for further analysis. Samples were stained using the routine hemalaun-eosin method and specific immunohistochemical staining for cytomegalovirus protein detection where antibody Croma 101, a monoclonal antibody specific for viral phosphoprotein pp89, was used as the primary antibody. Results: By microscopic analysis of the hemalaun-eosin stained samples of liver and salivary glands, changes can be detected by the viral activity, expressed in low intensity. By using immunohistchemical staining, more specificaly, viral induced changes have been detected due to the determination of the site of expression of the viral protein pp89. Conclusion: Changes induced by cytomegalovirus are significantly more difficult in the mouse of IFNαβR KO strain that is immunodeficient due to the lack of interferon receptors. In C57/B6 strain interferon receptors have influenced the suppression of infection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:428895
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-01-11 13:06:35