Objective: The aim of the study is to analyze the incidence of Helicobacter pylori in Osijek-Baranja County in a three-year period (2013 – 2015) by non-invasive immunocromatography, to determine whether there is a difference in the frequency of positive findings with regard to the age and sex and to analyze whether there is increase or decrease of incidence of Helicobacter pylori in the three-year period observed. Patients and Methods: Respondents were patients who, for suspicion of the infection with Helicobacter pylori, provided a stool sample stool to the Department of Microbiology of the Public Health Institute of Osijek-Baranja County from 2013 to 2015. During those three years 12 594 patients provided a sample for processing. Stool samples were tested with ''Diaqiuck'' tests produced by Dialab, based immunochromatographic assay. Results and conclusion: From 2013 to 2015, a total of 16 579 samples were tested for 12 594 patients. The number of analyzed stool samples was higher than the number of patients, as some of the patients provided more than one sample in order to determine successful bacterial eradication. The number of positive stool samples for Helicobacter pylori antigen is 4962, and the number of primo isolates is 3985. Positive Helicobacter pylori antigen was found more frequently in women (68% of positive Helicobacter pylori isolates) than in men (32% of positive Helicobacter pylori isolates). Generally, the largest number of positive Helicobacter pylori isolates was found in patients between 41 and 64 years of age (51.6%). From the analyzed data, it is evident that the number of primo isolates increases from year to year (2013 – 1251 positive isolates, 2014 – 1225 positive isolates, 2015 – 1509 positive isolates), and it can be said that the percentage of the total number of positive samples throughout the observed period, i.e. frequency is 25.7 %.