Objectives: The aim of this study was to research the most common pathogenic microorganisms in urine specimens from surgical Intensive Care Unit (ICU). To compare the therapy given based on clinical data and biochemical analysis with the therapy applied after the arrival of antimicrobial susceptibility results. Compare the number of patients with urinary tract infection in ICU with the total number of patients treated in ICU during that period and conclude whether urinary infection affects the final outcome of the treatment. Study design: Cross – sectional study Patients and Methods: The study included 237 patients hospitalized at ICU Osijek University Hospital in the period from 1st January 2018 to 31st December 2018, sampled for urine and urine culture. The data were obtained from the history disease analysis, the therapeutic lists and the 464 findings of urine and urine culture samples. Pathogens were identified, and for highly resistant pathogens finding antimicrobial susceptibility test was made. General patient data, comorbidities, inflammatory response parameters and attributed antimicrobial therapy were recorded. Results: Of the 237 patients, there were 154 men and 83 women. The average age of patients was 65.5 years, and the most patients at ICU were admitted from the Department of Abdominal Surgery. Of the 464 samples taken, 369 were sterile, while 95 were non-sterile. The number of uroinfections was 11.4 / 1000 days of catheterization. The most commonly isolated pathogenes were Candida albicans, Acinetobacter baumanii and Proteus mirabilis, mainly in elderly patients who stayed for a long time in ICU. Conclusion: Elderly patients, elevated CRP and body temperature values had statistically significant affect on the outcome of the treatment. The diagnose of uroinfection was not statistically significantly associated with the outcome of the treatment. Early antibiotic therapy was usually prescribed for prophylaxis or other infections. In cases of highly resistant pathogens it has been modified according to antimicrobial susceptibility.