The aim of the study was to investigate whether there is a significant difference in p2PSA values between control subjects and patients with prostate cancer and whether the PHI score is a more sensitive and specific indicator than % PSA for differentiating patients with benign changes from patients with cancer. Patients and Methods A total of 27 patients, who were classified into two groups according to the diagnosis, were included in the research: I) 11 patients with prostate cancer; II) 16 control subjects. Concentrations of PSA, fPSA and p2PSA were measured by chemiluminescent analysis on UniCel DxI 600 immunoassay analyzer (Beckman Coulter Inc., Brea, USA). The % PSA was calculated from the values of PSA and fPSA concentrations and all three parameters were used to calculate the PHI score. Statistical analysis of the results was done using MedCalc statistical software (MedCalc Software, Mariakerke, Belgium). ROC analysis was performed to test the sensitivity and specificity whereas the Mann-Whitney test was used for comparing the two groups. Results The study has not shown a significant difference in p2PSA values between patients with prostate cancer and the control subjects. With a cut-off level of 38,15, the PHI score has a higher sensitivity and specificity than the usually applied %PSA (AUC=0,767 vs AUC=676). Conclusion The study results have shown that the PHI score is a better parameter for assessing the risk of prostate cancer compared to the PSA index.