Objectives: The aim of this paper was to determine the socio-demographic characteristics, knowledge, attitudes and frequency of gambling of the student population of the University of Osijek and to research whether there is a correlation between the observed variables. Study Design: The research follows the principle of cross-sectional study. Participants and methods: A total of 844 students (54.4% female) in the first years of undergraduate and integrated and final years of graduate and integrated studies at the University of Osijek participated in the study. A literature search on gambling disorder in the student population was conducted via Web of Science. A part of this search was used to design a questionnaire. The questionnaire consisted of 42 questions which were sociodemographic, as well as questions on knowledge, attitudes and frequency of gambling. Results: The respondents are mostly unemployed and do not gamble to a large extent. A small number of respondents showed interest in gambling, which refers to: betting on sports and going to the casino, playing numbers or lottery, bingo, using slot machines, poker machines or other betting machines. Men are more prone to hiding betting tickets, lottery tickets etc. and also more prone to bet on sports. Students of social and technical sciences more often feel guilty because they gamble or because of what happens when they gamble, but are also prone to going to casino. Students who earn more money argue more often with people they live with because of the way they manage money. Conclusion: Most students don’t gamble into a greater extent. Knowledge and attitudes about gambling are good in most students, but not so much in students of social and technical sciences, as well as those who earn more compared to other students.