Objectives: The objectives of this study were to examine the incidence of ESCAPE pathogens in tertiary level hospital, to analyze their incidence considering departments and samples from which they were isolated, and finally to compare the incidence of resistant pathogens between the two three-year periods 2009 - 2011. and 2017 - 2019. in KBC Osijek. Material and methods: In this study, the annual Reports on the occurrence of resistant pathogens for 2009, 2010, 2011, 2017, 2018 and 2019 were used. The study included all patients and samples in which one of the studied multidrug-resistant bacteria was isolated; VRE, MRSA, C. difficile, A. baumannii, P. aeruginosa, Enterobacter spp. ESBL, E. coli ESBL and K. pneumoniae ESBL. Results: The highest incidence rates were recorded by isolates of A. baumannii, and the lowest by P. aeruginosa. In 2019, a total of 854 cases of resistant pathogens with 1218 samples were detected. Most in the department of infectology and ICU, from urine samples, airway aspirates, and stool. By comparing the incidence in the two three-year periods, it was found that K. pneumoniae and MRSA occur statistically significantly more common in the first (2009 -2011), and A. baumanni and E. coli in the second (2017 - 2019) observed three-year period. Conclusion: The growing trend of resistant isolates of A. baumanni and E. coli is in correlation with data from related recent research and the rapid growth in the number of nosocomial infections caused by these pathogens. The department of infectology and ICU, where the largest number of pathogens have been detected, due to their mostly immunocompromised patients, should be under special care in order to prevent the occurrence and spread of nosocomial infections.