OBJECTIVES: Objectives of this study were to determine cell viability of Huh7 cells at different concentrations of amiodarone, to measure the amount of cellular reduced glutathione (GSH) in Huh7 cells, as well as to visualize changes in morphology of cells treated with different concentrations of amiodarone using Oil red O staining. STUDY STRUCTURE: Randomized controlled trial. MATERIAL AND METHOD: The study was conducted on Huh7 cell line cell culture. Randomization was achieved by block design, and the experiments were repeated three times. Cells were treated with different concentrations of amiodarone after 48 hours of growth. After 24 hours, cell viability, proliferation and accumulation of lipids and neutral triglycerides were determined. RESULTS: Cell survival was significantly lower in the treated cell groups compared to the control group, but there was no statistically significant difference between the two treated groups. Measurement of the amount of cellular reduced glutathione (GSH) in Huh7 cells showed statistically significantly higher values in the control group compared to the 40 μM AMD and 20 μM AMD treated groups, and there was no statistically significant difference between the two treated groups. Oil red O staining showed significantly higher values in the amounts of fat droplets in the treated groups compared to the control, and statistically significantly larger fat droplets in the group treated with 40 μM AMD. CONCLUSION: Cell viability is significantly lower in the cell groups treated with 40 μM AMD and 20 μM AMD compared to the control group, while there is no statistically significant difference in cell survival between the two treated groups.. Significantly higher oxidative stress was suffered by the treated groups compared to the control. The treated groups also had more fat droplets, and the largest were in the group of treated cells of 40 μM AMD.