Objectives: To investigate the effects of intravenous thrombolysis in the treatment of stroke patients at Sveti Duh University Hospital during the period from 2007 to 2017. Objectives include analyzing trends in the number of thrombolysis, evaluating the quality and safety of treatment, and comparing the center's results with data from the international SITS registry for the same period. Study design: Cross-sectional study using historical sources. Participants and Methods: All patients aged 18 and older hospitalized ant treated with intravenous thrombolysis for acute stroke at the “Sveti Duh” University Hospital between 2007 and 2017. Results: A total of 227 patients with acute stroke who received systemic thrombolysis were included. The average age of the participants was 70 years. The largest percentage of patients (44%) had a cerebral infarction with large vessel disease, similar to the pattern in the entire Croatia (38%). Significant differences were observed in the time from hospital arrival to treatment initiation. The percentage of patients without symptoms or with mild symptoms after three months was 25%. The 7-day mortality rate was 6 - 8% in all three populations. The 3- month mortality rate increased to 11 - 15%. Conclusion: Patients who received systemic thrombolysis at "Sveti Duh" Hospital faced a more severe clinical presentation and multiple risk factors but achieved better long-term survival and independence outcomes. The number of thrombolysis increased during the study period, especially in 2013 and 2017. The time from arrival to treatment initiation at “Sveti Duh” University Hospital was the shortest. However, door-to-needle time was longer in Croatia in comparison to other SITS centers globally.