Research Objective: This study shows the incidence of tuberculosis in the Osijek-Baranja County, comparison of incidence in the same county with the incidence of tuberculosis in the Republic of Croatia, incidence of tuberculosis in epidemiological offices in the Osijek-Baranja County, comparison of incidence of tuberculosis according to age categories and distribution of tuberculosis cases regarding age and gender. Study Design: The data have been retrospectively analyzed during 19 years: from 1996 to 2014. Material and Methods: The study included 1979 examinees suffering from tuberculosis. Information about patients origin from the database of the Institute for Public Health of the Osijek-Baranja County. The Croatian Scientific-statistical yearbook for 2014 was used, as well as the 2001 and 2011 census. Retrospective analysis of data was carried out from the base of reported contagious diseases (tuberculosis), by age and gender and by area distribution within the Osijek-Baranja County. Results: During the study duration there appeared a negative incidence trend of tuberculosis. During the most years of the study duration, the incidence of TB in the Osijek-Baranja County was higher than the TB incidence in the Republic of Croatia. The highest incidence was reported in 1997 in the area of Valpovo office. The highest percentage of tuberculosis patients is found above the age of 60, most probably due to decrease of immune system of older persons and due to decrease of defence ability of the body against infections. Percentage of patients increased with attending of school. There are statistically significant more men than women suffering from tuberculosis. Conclusion: In order to eliminate tuberculosis he have to have a medical surveillance of patients, to perform a quality vaccination program against tuberculosis, and to improve the awareness about TB through health education within the community and among the TB patients. It is very important to maintain performing preventive measures more intensively.