The purpose of the study is to examine the degree of professional self-concept of nurses, to examine if there is a difference in self-concept in relation to work experience and to examine if there is a relationship between the length of work experience and professional self-concept of nurses. Scheme of study Research is established as cross-sectional study. Participants and methods The participants in the study were 135 nurses/ medical technicians of KBC Osijek, who work in Surgical Clinic, Neurology Clinic and Institute for dermatovenerology . The research was conducted from September to December 2016. For purpose of the study anonymous questionnaire was used in order to determine professional self-concept based on Likert scale. The observed subscales were leadership, flexibility, skills, pleasure, and communication. Results The results show the highest agreement with statements in relation to flexibility with skills domain. Leadership domain has an average value. There is a significantly higher agreement of participants, in leadership domain, who are more than 50 years old. In other scale domains, as well as total scale there are no statistically significant differences concerning age. There are no statistically significant differences in agreements with statements of total scale in relation to length of work experience. Age and length of work experience are weakly but significantly related to total scale of self-concept of nurses/technicians. There is a weak but significant relationship between age and flexibility, leadership and skills. A strong relationship is between the length of work experience and skills. Participants who gave higher grade to flexibility gave also significantly high grade to skills and leadership. A significant relationship is between leadership and skills. There is a weak but significant relationship between leadership and pleasure. Conclusion In conclusion, there is no statistically significant difference of total scale in relation to age or length of work experience. There is a significantly higher agreement of participants, in leadership domain, who are more than 50 years old. There is a weak but significant relationship between age and work experience.