OBJECTIVE: The objective of this paper is to determine the differences in knowledge and opinions about oral hormonal contraception among first and third year students of nursing at the undergraduate nursing studies in Osijek. STUDY DESIGN: Cross – sectional study. PARTICIPANTS AND METHODS: The study included first and final year female students of nursing. The method of research was an anonymous questionnaire, which was created in collaboration with the mentor. RESULTS: 79 participants – first and third year female students participated in this research. A large number of first year students (70 %), know that occasional breaks in the use of oral hormonal contraception shouldn’t be made, while (55 %) of third year students aren’t aware of that fact. Most first year students (52 %) know the benefits of using oral hormonal contraception, which cannot be said for the third year students. A high percentage of both first (70 %) and third year students (77 %) don’t use oral hormonal contraception. When it comes to female knowledge on oral hormonal contraception, most first year students (52 %) think women are not properly informed, while the majority of third year students (77 %) believe that women posses enough information. CONCLUSION: Most first and third year students showed that they posses enough information on menstrual cycle, methods of contraception and the significance of oral hormone contraceptives for women's health. The study has shown that first year students know more about oral hormonal contraception. Third year students visit their gynecologists more often, while first-year students think that, in Croatia, women are not properly informed about oral hormonal contraception.