Objectives: To examine knowledge and opinion among parents of highschool children in Brodsko-posavska county about human papillomaviruse vaccine Study Design: Cross sectional study Participants and methods: The subjects were 189 parents of pupils in a first and second grade in Nursing school and Trade school in Slavonski Brod. Instrument used in the study was a self-administered anonymous questionnaire made especially for this survey based on a previously used questionnaire (Septimiu Voidăzan, 2016.) with the permission of the author. Results: Of the respondets, 90 % have heard of HPV infection. Significantly more parents with high school and college education know that vaccination prevents genital warts, anus cancer and cervical cancer. For the subjects of the Trade School, the main source of information on HPV infection and HPV vaccination are healthcare professionals, and secondly, the Internet, while for Nursing school respondents, the Internet is the first and the second are healthcare professionals. Although as many as 77 % of the respondents believe that vaccination is necessary, only 9 % of them have vaccinated their child without statistical difference between the school attendance or the level of education. As a reason why they did not vaccinated significantly more subjects whose children go to Trade School state that they do not know about vaccination against HPV, while significantly more parents of children in the Nursing School are afraid of the side effects of the vaccine. Significantly more respondents who have only primary education are not aware of the existence of the vaccine. Conclusion: The parents of students who attend Trade school have significantly less knowledge on human papillomavirus infection and vaccination against HPV. Significantly more parents with high school and college education have shown greater knowledge about HPV infection and HPV vaccination. The most common reasons for not wanting to have their child vaccinated is the fear of side effects, lack of awareness of the existence of the vaccine, and insufficient vaccine research.