Introduction: Obesity is the risk factor for the development of type II diabetes. Diabetes is a disease characterized by hyperglycemia, insulin resistance and chronic inflammation. Accordingly, the metabolism of iron in the liver and brain changes in the body and tissue damage occurs. Using pre-diabetic animals, it is possible to monitor the pathophysiological changes and the effect of the drugs on them. Goals: In 4 groups of animals on the 1. standard diet (SD), 2. diet enriched with fats and sugars (HFHSD), 3. enriched diet and then treated with antidiabetic metformin (HFHSD-M) and 4. liraglutide (HFHSD-L) to evaluate the iron content in the liver and region of substantia nigra (SN) by using the Perls method, and in the SN region by immunohistochemical localization of the transferrin receptor. Immunohistochemically evaluate regenerative liver function. To evaluate the amount of amyloid in the SN region using histological color Congo red, and the number of neurofibrillary bundles immunohistochemically. Materials and methods: The study included 32 female Sprague Dawley rats divided into 4 groups: 1. SD, 2. HFHSD, 3. HFHSD-M and 4. HFHSD-L. With histological staining Perls method and Congo red we detected iron and amyloid. Immunohistochemically, using transferrin receptor antibodies, phosphorylated Tau (pTau), neural nitric oxide synthase (nNOS), and core proliferating antigen (PCNA), respective markers were detected. Results: There is a statistically significant difference between iron staining results and PCNA antigen, while in the case of pTau, nNOS and Congo staining, no statistically significant difference was found. The antidiabetics metformin and liraglutide have partially managed to show their therapeutic effects. Conclusion: In the pre-diabetes, disorders of iron metabolism in the liver and brain and tissue damage occur.