Introduction: Cell membrane consists of phospholipid double layer that is thicker packed and less fluid in some places. These parts are called lipid rafts. Lipid rafts, except proteins, cholesterol and lipids, are built of gangliosides which are present in all tissues, but they are the most numerous in the brain. Their disorder leads to neurodegeneration, various disorders and tumors, including the most agressive, hard to cure, glioblastoma. Goals: To determine the total composition of gangliosides and to determine present complex gangliosides in glioblastoma cell lines. Materials and methods: For this study two glioblastoma cell lines (CRL-1620 i HTB-15) were used. After cultivation, it was needed to extract gangliosides from the cells. Then the total gangliosides were analysed using the TLC method. In order to determine present gangliosides, immunochemical analysis of the cells is made using highly specific, mouse monoclonal IgG antibodies. The results were then analyzed using a flourescent microscope and were photographed by camera. The created images were made in computer program CorelDraw X3. Results: The difference between two (glioblastoma)* cell lines in composition of gangliosides obtained by the method immunocytochemistry is present. In glioblastoma cell line CRL-1620 the most abundant ganglioside is GD3, while in glioblastoma cell line HTB-15 are equally poorly presented GD1a, GD1b and GD3. Using the TLC are detected GM3 and GM2 in both cell lines and GD3 in glioblastoma cell line CRL-1620. Conclusion: Phenotypically different glioblastoma cell lines differ in the composition of gangliosides, but in both analyzed cell lines complex gangliosides are present in very small amounts on none at all. The most important gangliosides that are overexpressed are GD3, GM2 and GM3.