Aims: The main goal of the study was to increase the consumption of first-line antibiotics in the treatment of uncomplicated cystitis. Educational intervention in the form of a review article with case reports was designed to increase the consumption of first-line antibiotics, as well as to improve the consumption parameters in accordance with the ESAC quality indicators for antibiotic prescribing. Methods and subjects: The study was organized as a prospective intervention study with the participation 42 family physicians. The data on the number of visits related to urinary tract infection diagnoses and issued antibiotic prescriptions were collected from medical computer programs, based on monthly reports. All physicians were considered as a single sample, and the examination of the effectiveness of the intervention (consumption of antibiotics before and after the intervention) was performed by methods of analyzing the paired sample. Results: A total of 67 547 people were in the care of doctors, of which 31 214 were men and 36 333 women. A total of 3581 prescriptions were prescribed for uncomplicated cystitis in women over the age of 18, of which 1717 before and 1864 after education. Consumption increased by 147 prescriptions (8.5 %). The number of prescriptions for first-line antibiotics (nitrofurantoin, fosfomycin and trimethoprim-sulfamethoxazole) increased from 837 to 1015 (178 prescriptions or 21.2 %), the number of prescriptions for fluoroquinolones decreased from 332 to 310 (6.6 %), while the use of beta-lactams remained unchanged (30.3 %). After the intervention, nitrofurantoin was the most prescribed of the first-line antibiotics (178 prescriptions, or 21.2 %). The total volume of consumption after the intervention, in terms of quality indicators, was 100 % for indicator “a”, 54 % for indicator “b”, and 16.6 % for indicator “c”. Conclusion: Following the intervention, family physicians increased their prescribing of first-line antibiotics, mostly nitrofurantoin. The difference in the change in the number of prescriptions before and after the educational intervention was statistically significant. Despite the increase in the consumption of first-line antibiotics (48.7 % before and 54.4 % after the intervention), the acceptable ranges did not reach the values predicted by the ESAC quality indicators.