Objectives: Using mass spectrometry imging to try to determine if there is a disturbance in the lipid metabolism of the brain regions of mice with St8sia1 gene turned off (knock-out, KO) compared to wild type mice (WT) of the same age. Define lipidoma metabolites whose metabolism has been altered. Confirm the results with histological staining methods. Study design: Study of pairs Materials and methods: Two groups of mice were used: KO and WT. 8 male mice, 4,5 months old, 4 mice of each genotype were used. Imaging mass spectrometry and the following histological staining were performed on fresh tissue sections: alkaline phosphatase enzyme activity, succinate dehydrogenase enzyme activity, cholesterol, phospholipid tissue distribution, and actin tissue distribution. Quantification of histological staining was done using a computer program Fiji. Results: Mass spectrometry shows a clear difference between KO and WT mice by analyzing the basic components. A statistically significant change in metabolites was found on the nucleus caudatus et putamen region in different metabolic pathways. Staining of the enzymatic activity of succinate dehydrogenase showed a significant reduction in staining in the capsula interna region. By staining the tissue distribution of cholesterol, a weaker staining of the capsula interna and nucleus caudatus et putamen regions was found, and by staining of phospholipids, a weaker staining was found in all regions. In contrast, actin microfilament staining was significantly enhanced in all regions of KO mice. Conclusion: The results suggest that KO mice have changes in brain lipidoma that could have functional consequences.