Objectives: The aim of this study was to measure the concentrations of aspartame in refreshing soft drinks and compare them with the maximum allowable concentration and with concentrations on the nutrition labels. Also, the aim was to examine the frequency of consumption of soft drinks, but also the incidence of Internet addiction in the student population in Osijek. Study design: Cross-sectional study. Participants and Methods: In the first part of the study, concentration of aspartame was measured in 28 samples of soft drinks by HPLC/DAD method. In the second part of the research, a survey among the students at the University of J. J. Strossmayer Osijek was conducted. The questionnaire contained 38 questions of socio-demographic and socio-economic status, but also questions about consumption of soft drinks and questions about circumstances of the Internet use. Among the 792 examinees, 473 were females and 319 were males. Results: The median of measured concentrations of aspartame was 22,70 miligrams per liter, which is in accordance with the Regulation on food additives in Europe. The highest concentrations were measured in artificial soft drinks as opposed to fruit juices. 85,70 % of students consume soft drinks, on average 0,20 liter per day. 60,20 % do not have an Internet addiction, while 0,50 % have a severe Internet addiction. Conclusion: All measured concentrations of aspartame were in accordance with the maximum allowable concentrations. Male students (90,30 %) more often consume soft drinks than females (82,70 %). There is no statistically significant association between the frequency of consumption of soft drinks and the occurence of Internet addiction among student population in Osijek.