Research aim: The aim of this research was to determine and compare morphometric and biomechanics characteristics of distal superficial third of quadriceps muscle tendon and distal tendon of gracilis muscle, as well as compare those results with morphometric and biomechanical results of medial patellofemoral ligament (MPFL) acquired from available literature. Research plan: The research design of this study was cross-sectional study of 34 gracilis muscle tendons and 34 superficial distal tendons of quadriceps muscle with in advance determined biomechanical features. Materials and methods: Research material belongs to the archives of Department for Anatomy and Neuroscience, Faculty of Medicine Osijek, J. J. Strossmayer University of Osijek. Patients with knee joint injury or collagenosis were excluded from the study. Male and female samples of 34 gracilis muscle tendons and 34 distal superficial quadriceps muscle tendons were acquired between years 2000 and 2015. Median age of the included patients was 71 years. Special device constructed in Mechanical Engineering Faculty in Slavonski Brod was used to examine tendons and data analysis. After standardized load on the tendons, the elongation force, tensile strength, extensibility, stiffness, and elasticity module were measured. All those results were compared to studies containing data about MPFL’s biomechanical features. Results: Cross sectional area as well as tendon width were significantly higher in quadriceps than gracilis muscle tendon. Tendon length and thickness were significantly higher in gracilis muscle tendon compared to quadriceps tendon. There was no significant difference in maximal force between gracilis and quadriceps tendons. Elongation, wave strength, extensibility, and elasticity module were significantly higher in gracilis tendon than in quadriceps tendon. Maximal and minimal strain relaxation in 3600 s was significantly higher in gracilis tendon than in quadriceps muscle. Maximal strain time was significantly higher in gracilis tendon than in quadriceps one. Considering the obtained results, quadriceps tendon reacts much better to the load than gracilis tendon and therefore the strain on it is much smaller. In other words, quadriceps tendon provides greater resistance which shows a longer time needed to achieve strain when compared to gracilis tendon. Conclusion: Biomechanical characteristics of distal superficial third of quadriceps tendon are more suitable than those of gracilis tendon and could be useful in operating techniques of MPFL repair.