Objectives: To examine the frequency of carbapenem-resistant species of the order Enterobacterales in a tertiary hospital centre, their sensitivity to antibiotics and the frequency of production of certain types of carbapenemases. Study design: cross-sectional study Materials and methods: Data on samples of hospitalized patients in the period from January 1, 2021 to December 31, 2022 were analysed. In the identification of bacterial species, a MALDI-TOF mass spectrometer was used with the MALDI Bruker Biotyper base and IVD commercialized platform for identification and sensitivity testing to antibiotics BD Phoenix M50. In sensitivity testing, the standardized disk-diffusion method according to Kirby-Bauer and the e-test method according to the EUCAST methodology were used. Detection of carbapenemases was performed using the commercial immunochromatographic test RESIST-% O.K.N.V.I. Statistical methods used in data processing are χ² test and Fisher's exact test. Results: In a two-year period, 128 strains of carbapenem-resistant enterobacteria were isolated, most of which were strains of K. pneumoniae (85.9%). The strains were most often isolated from urine samples (42.2%). OXA-48 carbapenemase was detected in 81.3% of isolates. The sensitivity of the tested isolates was the highest in the case of cefiderocol (96%) and ceftazidime-avibactam (94.4%), and the lowest in the case of meropenem (31.3%) and tigecycline (35.2%). The increase in the sensitivity of carbapenem-resistant enterobacteria to colistin and tigecycline during 2022 compared to 2021 proved to be statistically significant. Conclusion: 128 strains of carbapenem-resistant enterobacteria were isolated, the largest share being K. pneumoniae strains. The most prevalent mechanism of resistance to carbapenems is the production of OXA-48 carbapenemase. The most isolates were sensitive to ceftazidime-avibactam and cefiderocol, and the least to meropenem and tigecycline.