OBJECTIVES: To examine the correlation between the age of patients and the diagnosis of prostate cancer, the association of a positive clinical examination (digital rectal examination) with the diagnosis of prostate cancer, and the relationship between positive mpMRI findings of the prostate and the diagnosis of prostate cancer. Additionally, to determine the number of prostate biopsies that resulted in a diagnosis of prostate cancer. STUDY DESIGN: A cross-sectional study using historical data. PARTICIPANTS AND METHODS: Based on the inclusion criterion (performed prostate biopsy), the study included participants who underwent the mentioned procedure between January 1, 2023, and December 31, 2023. Clinical and laboratory data were collected and analyzed from hospital records. RESULTS: A total of 343 prostate biopsies were performed on 339 patients, as four patients required a re-biopsy within a one-year period. Patients with malignant histopathological findings were significantly older (median age 68 compared to 66 years). Prostate cancer was confirmed in 52.2% of cases. According to localization, 99 patients had cancer in both lobes. The most common Gleason score was 3 + 4 = 7, found in 34.1% of patients. According to the ISUP classification, 34.1% of patients were in Grade 2, while 24.6% were in Grade 4 or 5. The median PSA value for all biopsies was 8.89 ng/mL. One-third of the patients underwent mpMRI before the biopsy, with 40 of them receiving a PI-RADS score of 3. ASAP was found in 10.2% of prostate biopsies, and PIN in 40.2%. CONCLUSION: Patients diagnosed with prostate cancer were older and had abnormalities on DRE, while every other biopsy resulted in a malignant finding