Objective: The objective of this research is to evaluate elementary school teachers' knowledge and opinions of type 1 diabetes in children and examine whether there are differences in knowledge and opinions of 1st to 4th grade teachers as opposed to 5th to 8th grade teachers. Study Design: The study was conducted as cross-sectional. Participants and methods: The participants, a total of 43, were teachers employed in elementary schools in Osijek. The research was conducted in June 2017. A modified standardized questionnaire for assessing the elementary school teachers' knowledge and opinions of type 1 diabetes in children was used as a research instrument. The data was processed in SPSS program (version 16.0, SPSS Inc., Chicago, IL, USA). Results: There is a marginal significance in claiming that higher amounts of sugar in the diet cause type 1 diabetes (p = 0.062), and 60% of teachers think it is correct. More teachers, 30% of them, think that blood glucose levels are low in children with undiagnosed type 1 diabetes (p = 0.065). Statistically significantly more teachers, 38.2%, think that the loss of consciousness is a symptom of diabetes as compared to the opinions of 5.26% of teachers (p = 0.020). Also, more teachers consider that paleness is the symptom (p = 0.029). Conclusion: The total knowledge and opinions of elementary school teachers is insufficient. The teachers have different knowledge and opinions about the cause and the recognition of the symptoms of the disease.