OBJECTIVE: The objectives of the study were to determine whether there is any difference in the measurement of intraocular pressure by Goldmann aplanatine tonometry and air-puff tonometry and to compare the feeling of comfort when measuring the intraocular pressure by both methods. STUDY DESIGN: cross-sectional study PARTICIPANTS AND METHODS: The study included 133 participants, who underwent standard ophthalmic examination. Participants were examined for correction of refractive error from October 2017 to April 2018 at the Ophthalmology polyclinic „Oculus“ in Osijek. Exclusion criteria were: age under 18 years, glaucoma treatment, operative surgery at the anterior or posterior eye segment, degenerative changes in the anterior eye segment or tear film disorder. Intraocular pressure was measured in all participants by both methods, after which participants voluntarily completed a questionnaire in which the following data were collected: age, gender, data on wearing lenses, number of IOP measures and evaluation of the comfort level of Goldmann aplanatine tonometry and air-puff tonometry. RESULTS: This cross-sectional study included 133 patients (83 women, 50 men). Median of the participants' age was 51. There was statistically significant difference between the measured intraocular pressure of the right and left eye with the Goldmann aplanatine tonometer and air-puff tonometer (t-test, P<0.001). There was no significant difference in the comfort level of intraocular pressure measured with Goldmann aplanatine tonometer and air-puff tonometer (Fisher's exact test, P=0.18). CONCLUSION: Although air-puff tonometry has some advantages over Goldmann aplanatine tonometry, Goldmann aplanatine tonometry provides more accurate results for intraocular pressure measurement and is therefore considered a gold standard for IOT measurement today.