Introduction: The most common chronic complication in patients with diabetes mellitus is diabetic nephropathy. Hyperglycemia and hemodynamic changes in glomeruli are responsible for the development of diabetic nephropathy. Diabetic nephropathy is one of the main risk factors for the development of cardiovascular diseases and it increases the risk of mortality from these diseases. Albuminuria, hypertension and reduced glomerular filtration rate are characteristic for this disorder. Albuminuria is mostly diagnosed in patients with diabetes or hypertension. Objectives: To evaluate the degree of renal impairment based on the measured urine albumin level. Study Design: Cross-sectional study Materials and methods: The study included 60 respondents, 30 healthy and 30 diabetic patients. For the purposes of this study, blood and urine samples were collected at the Department of Clinical Laboratory Diagnosis within Osijek Clinical Hospital Center. The glucose concentration was determined on the Beckman Coulter AU680 instrument, using the enzyme method with hexokinase. Immunoturbidimetric method is used to determine HbA1c in the blood sample on the Siemens Dimension ExL instrument. The same method was used to determine albumin in the urine sample, but on the Beckman Coulter AU680 instrument. Results: Measured blood glucose and HbA1c values and albumin in urine were significantly increased in diabetics compared to the control group, measured in Mann-Whitney U tests. (P <0.001 for all three measured values). Also, significantly more diabetics have a positive urinary glucose concentration compared to the control group (P <0.001), as measured by Fisher's exact test. . Conclusion: Patients with type II diabetes have higher concentrations of urinary albumin, which would suggest that the kidney damages was caused due to long-lasting hyperglycemia.