Aim of research: Examine the attitudes of Čakovec High School students (Srednja škola Čakovec) towards people of homosexual orientation, compare the attitudes of female students to those of male students towards people of homosexual orientation, compare the attitudes of medical students to those of grammar-school students towards people of homosexual orientation, and compare the attitudes of final grade students to those of students at the beginning of their secondary education towards people of homosexual orientation. Respondents and methods: A cross-sectional study has been conducted including 338 students attending Čakovec High School (Srednja škola Čakovec) medical and grammar school programme. An anonymous poll sheet has been used as an instrument of research, which consisted of five parts. The questionnaire consisted of 41 closed-ended questions. Results: Research results have shown that students have a positive attitude towards people of homosexual orientation. There is a significant difference in the attitudes towards people of homosexual orientation with regard to gender, where male students have shown more negative attitudes in comparison to female students. Grammar school students have more knowledge of homosexuality than medical students, and they also have more positive attitudes towards people of homosexual orientation than medical students. Conclusion: Research results prove that having more knowledge about people of homosexual orientation leads to better understanding and more positive attitudes towards them. High-school students do not get enough information about people of homosexual orientation, which leads to the stigmatisation of such people and the creation of negative attitudes towards them.