The objective: The objective of this paper work is to study the connection between reference values of blood and urine tests and newly formed osteoporotic fractures of vertebral body. The success of the research will be defined by establishing the connection between significant change in blood test values and the occurrence of vertebral fractures among the population of men participating in the research. The outline of the study: The research was conducted as an average study on 36 male subjects ranging from 50 to 84 years old. Subjects and methods: The information about the male patients was collected from the Central Information System of Department of orthopaedics and trauma at the University Hospital Centre in Osijek. The study included 36 male patients who have suffered spinal fracture in the last six months and who have done standardized laboratory blood and urine tests before or immediately after the vertebroplasty. The study analysed reference blood and urine values and their association with the number and position of the fracture as well as the risk of new osteoporotic vertebral fractures. Results: Considering the location of the fracture, 8 (22%) research subjects suffered a fracture of thoracic, 17 (47 %) of lumbar and 11 (31 % )of both thoracic and lumbar spine. There was a larger number of fractures among the patients with lumbar and thoracic spinal fractures. The study showed lower values of haemoglobin with research subjects with lumbar fractures whereas there was no significant change in other biochemical analysis results. Furthermore, there was a visible connection between the level of phosphates in the urine and the number of fractures. Among the research subjects who had both thoracic and lumbar fracture, there was a connection between the number of fractures and ALT values. Conclusion: After analysis and processing of research data, it is concluded that there is no significant connection between the reference values of blood and urine laboratory tests and the risk of vertebral fracture.