Objectives: The goal of this study was to assess knowledge, attitudes and opinions about breast cancer prevention medications among women without diagnosed breast cancer after attending a routine breast ultrasound or mammography at the Department of Ultrasound Diagnostics and Mammography at the Health Center Osijek. Study design: A cross - sectional study. Participants and methods: A 54 - item questionnaire, divided into 4 sections, was constructed for the purpose of the study. Participants reported their breast cancer 5 - year and lifetime worry and self - perceived 5 - year and lifelong risk (absolute and relative to other women of the same age) of breast cancer using a 5 - point Likert scale. Participants' breast cancer risk was estimated based on The Breast Cancer Risk Assessment Tool (BCRAT). Knowledge about breast cancer risk factors was assessed trough 15 items. Knowledge of chemopreventative agents used in breast cancer, attitudes about chemoprevention, preference in the route of administration (topical/oral) and side effects worry were tested using 5 - point Likert scales trough 19 items. Sociodemographic, health and lifestyle - related characteristics were assessed as well. Results: Participants (n=178; n=164 after applying exclusion criteria) were divided into 3 groups based on the accuracy of their 5 - year risk self - evaluation. Perceived risk was defined as accurate (n=110) if the participant with a BCRAT 5 - year risk ≤1,7 % self - assed the risk as "very low", "low" or "average", or if a participant with a risk of >1,7% answered with "high" or "very high". Underestimation group (n=37) was defined with BCRAT risk >1,7 % and answers "very low", "low" or "average". Overestimation group (n=13) was defined by BCRAT risk ≤1,7 % and answers "high" or "very high". Conclusion: Raising awareness of chemopreventive options for breast cancer risk reduction may facilitate informed decision about preventative chemotherapy utilization among women with high risk of developing breast cancer.