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master's thesis
Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli
2019. urn:nbn:hr:152:445517
Tomac, Petra
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Tomac, P. (2019). Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:445517

Tomac, Petra. "Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:445517

Tomac, Petra. "Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:445517

Tomac, P. (2019). 'Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 March 2024, https://urn.nsk.hr/urn:nbn:hr:152:445517

Tomac P. Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 March 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:445517

P. Tomac, "Promjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:445517

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TitlePromjena proteinskog izražaja antioksidativnih enzima u krvnim žilama mozga Sprague-Dawley štakora primjenom subpresorskih doza angiotenzina II kod unosa visokih koncentracija soli
Title (english)Changes in protein expression of antioxidative enzymes in brain blood vessels of the Sprague-Dawley rats using suppressor doses of angiotensin II by intake high concentration of salt
AuthorPetra Tomac
MentorAnita Matić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberAnita Matić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Abstract
CILJ ISTRAŽIVANJA: Cilj je ovog istraživanja ispitati promjenu proteinskog izražaja važnih antioksidativnih enzima u krvnim žilama mozga muških Sprague-Dawley štakora hranjenih visoko slanom dijetom uz dodatak subpresorskih doza angiotenzina II. NACRT STUDIJE: Eksperimentalna studija na pokusnim laboratorijskim životinjama ISPITANICI I METODE: Zdravi muški Sprague-Dawley štakori, starosti 10 tjedana, nasumično su podijeljeni u tri eksperimentalne skupine (N = 8 - 9): niskoslana skupina (NS skupina, koja je konzumirala 0,4 % NaCl u hrani za štakore) te dvije visokoslane skupine (VS) koje su konzumirale specijalnu hranu s 4 % NaCl-a u svom sastavu tijekom 7 dana. Jednoj od slanih skupina zadnja tri dana protokola je ugrađena pumpa subpresorske doze angiotenzina II (100 ng/kg/min/3 dana). Nakon dijetnog protokola životinje su bile izvagane, anestezirane kombinacijom ketamina (75 mg/kg) i midazolama (2,5 mg/kg) te žrtvovane dekapitacijom. Prikupljene su krvne žile mozga u svrhu određivanja proteinskog izražaja antioksidativnih ezima (Cu / Zn SOD, MnSOD, EC-SOD, GPx4 i katalaze (CAT)) metodom Western blot. REZULTATI: Proteinski izražaj antioksidativnog enzima GPx4 značajno je snižen u skupinama VS [0,44 (0,24)] i VS+ANG II [0,38 (0,19)] skupinama (p > 0,05) u odnosu na NS [1,03 (0,46)] skupinu. Izražaj drugih antioksidativnih enzima nije se značajno promijenio između ispitivanih skupina (p < 0,05). ZAKLJUČAK: Povećana razina oksidativnog stresa u visokoslanoj skupini posljedica je sniženog izražaja GPx4 antioksidativnog enzima, dok pozitivan efekt angiotenzina II na snižavanje oksidativnog stresa i povećanje razine antioksidativnih enzima nije utvrđen na proteinskoj razini, subpresorske doze angiotenzina II ne dovode do značajne promjene izražaja antioksidativnih enzima na proteinskoj razini.
Abstract (english)
RESEARCH OBJECTIVE: The aim of this study was to assess the change in the protein expression of important antioxidant enzymes in the brain blood veins of male Sprague-Dawley rats under high salt intake and angiotensin II subpressor dose administration. STUDY DESIGN: Experimental study on laboratory animals MATHERIALS AND METHODS: Healthy male Sprague-Dawley rats, aged 10 weeks, were randomly divided into three experimental groups (N = 8 - 9): low-salt group (LS group, which consumed 0,4 % NaCl in rat food) and two high-salt groups (HS) which consumed special food with 4 % NaCl for 7 days. In the last three days of the protocol a subpressor dose of angiotensin II (100 ng / kg/ min/ 3 days) pump was included to one of the saline groups. After the dietary protocol, the animals were weighed, anesthetized by ketamine (75 mg / kg) and midazolam (2,5 mg / kg) and sacrificed by decapitation. Brain blood vessels were collected for determining the protein expression of antioxidative enzymes (Cu / Zn SOD, MnSOD, EC-SOD, GPx4 and Catalase (CAT)) by Western blot method. RESULTS: Protein expression of the antioxidant enzyme GPx4 was significantly decreased in high salt [0,44 (0,24)] and high salt+angiotensin II group [0,38 (0,19); p > 0,05] compared to low sat group [1,03 (0,46)]. The expression of other antioxidant enzymes did not change significantly between the investigated groups (p < 0,05). CONCLUSION: Increased level of oxidative stress in the high salt group is the result of the lower expression of the GPx4 antioxidant enzyme while the positive effect of angiotensin II on lowering oxidative stress and increasing the level of antioxidative enzymes is not established at the protein level, subpressor doses of angiotensin II do not result in significant changes in the expression of antioxidant enzymes at the protein level.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:445517
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-10-25 11:43:34