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master's thesis
Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji
2021. urn:nbn:hr:152:296538
Poštić, Mirna
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Poštić, M. (2021). Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:296538

Poštić, Mirna. "Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:296538

Poštić, Mirna. "Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:296538

Poštić, M. (2021). 'Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:296538

Poštić M. Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:152:296538

M. Poštić, "Uloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:296538

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Metadata
TitleUloga ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora nakon akutnog izlaganja hiperbaričnoj oksigenaciji
Title (english)The role of ion channels in the mechanisms of vascular reactivity in Sprague-Dawley rats after acute exposure to hyperbaric oxygenation
AuthorMirna Poštić
MentorIvana Jukić (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Istraživanje je za cilj imalo ustanoviti ulogu BKCa i KATP ionskih kanala u mehanizmima vaskularne reaktivnosti kod Sprague-Dawley štakora. Materijali i metode: U istraživanje su bili uključeni zdravi muški Sprague-Dawley (SD) štakori podijeljeni u dvije skupine: 1. skupinu su činili kontrolni, netretirani zdravi štakori (CTRL, N=12), a u 2. su skupini bili štakori podvrgnuti akutnoj hiperbaričnoj oksigenacijj (100% O2, 2h/2bara) (A-HBOT, N=12). Po završetku HBOT, štakori su bili anestezirani ketaminom (75 mg/kg) i midazolamom (0,5 mg/kg), te je uslijedila dekapitacija i izolacija središnje moždane arterija – modela na kojem se određivala protokom potaknuta dilatacija bazalno i u prisutnosti agonista ionskih kanala (BKCa agonist i KATP agonist), pri različitim gradijentima tlaka. Rezultati: Protokom potaknuta dilatacijaznačajno je smanjenjau A-HBOT skupini u odnosu na CTRL skupinu. U CTRL skupini agonist BKCaznačajno su doveli do povećanja FID-a samo pri višim vrijednostima tlaka (Δ60 mmHg i Δ100 mmHg), dok agonist KATP kanala nije utjeca na FID. Primjena agonista BKCai KATP kanala u A-HBOT skupini značajno je povećala protokom potaknutu dilataciju pri svakom gradijentu tlaka, u odnosu na bazalni odgovor bez primjene agonista. Zaključak: Akutna hiperbarična oksigenacija značajno smanjuje protokom potaknutu dilataciju, a BKCa i KATP kanali imaju značajnu ulogu u mehanizmu protokom potaknute dilatacije.
Abstract (english)
Objectives: The study aimed to establish the role of BKCa and KATP ion channels in the mechanisms of vascular reactivity in Sprague-Dawley rats. Studydesign: Experimental study on laboratory animals. Materials andmethods: The study included healthy male Sprague-Dawley (SD) rats divided into two groups: group 1 consisted of control, untreated healthy rats (CTRL, N = 12), and group 2 included rats subjected to acute hyperbaric oxygenation (100 % O2, 2x / 2bar) (A-HBOT, N = 12). After hyperbaric oxygenation therapy, rats were anesthetized with ketamine (75 mg / kg) andmidazolam (0.5 mg / kg), followed by decapitation and isolation of the middle cerebral artery – a model on which flow-induced dilation (FID) was determined – basal and in the presence of ion channel agonists (BKCa agonist and KATP agonist), at different pressure gradients. Results: Flow-induced dilatation is significant reduced in the A-HBOT group compared to the CTRL group. In the CTRL group, BKCa agonists significantly led to anincrease in FID only at higher pressure values (Δ60 mmHg and Δ100 mmHg), while the KATP channel agonist did not affect FID. In the A-HBOT group BKCa and KATP channel agonists significantly increased the flow-induced dilation at each pressure gradient, compared with basal response without the agonists. Conclusion: Acute hyperbaric oxygenation significantly reduces flow-induced dilatation, and BKCa and KATP channels play a significant role in the flow-induced dilatatio nmechanism.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:296538
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-03-06 12:48:49