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Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E
2022. urn:nbn:hr:152:707532
Grinžek, Tajana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Grinžek, T. (2022). Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:707532

Grinžek, Tajana. "Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:707532

Grinžek, Tajana. "Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:707532

Grinžek, T. (2022). 'Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 October 2024, https://urn.nsk.hr/urn:nbn:hr:152:707532

Grinžek T. Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 October 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:707532

T. Grinžek, "Izražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:707532

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TitleIzražaj mRNA enzima COX1, COX2, eNOS i iNOS kod sportaša koji konzumiraju kokošja jaja obogaćena n-3 polinezasićenim masnim kiselinama, luteinom, selenom i vitaminom E
Title (english)mRNA expression of enzymes COX1, COX2, eNOS and iNOS in athletes consuming hen eggs enriched with n-3 polyunsaturated fatty acids, lutein, selenium and vitamin D
AuthorTajana Grinžek
MentorInes Drenjančević (mentor)
Committee memberIvana Jukić (predsjednik povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
CILJ ISTRAŽIVANJA: Cilj je bio ispitati učinke konzumacije kokošjih jaja obogaćenih n-3 PUFA, luteinom, selenom i vitaminom E na enzime uključene u proizvodnju vazoaktivnih metabolita (COX1, COX2, eNOS, iNOS) NACRT STUDIJE: Istraživanje je ustrojeno kao randomizirano, dvostruko slijepo i placebo kontrolirano. ISPITANICI I METODE: Kontrolirana, intervencijska studija (Clinical Trials ID: NCT04564690) provedena je na zdravim mladim sportašima (N = 14) koji su konzumirali 3 kokošja jaja dnevno tijekom 3 tjedna. Nutri4 skupina (N = 8) konzumirala je obogaćena jaja (n3-PUFA: 1026 mg/dan, lutein: 616 mg/dan, selen: 0,02305 mg/dan i vitamin E: 1098 mg/dan), a kontrolna skupina (N = 6) konzumirala je obična jaja (n-3 PUFA: 438 mg/dan, lutein: 110 mg/dan, selen: 0,0138 mg/dan, vitamin E: 595 mg/dan). Uzorci krvi su prikupljeni prije i nakon konzumiranja jaja. Ekspresija mRNA COX1, COX2, eNOS i iNOS enzima u leukocitima periferne krvi mjerena je qPCR metodom (BioRAD, CFX96 Real-Time System), normalizirana na GAPDH housekeeping gen. ANOVA je provedena za statističku analizu s Tukeyjevim post hoc testom; p<0,05 smatra se statistički značajnim. REZULTATI: Konzumacija obogaćenih kokošjih jaja nije dovela do značajnog povećanja genskog izražaja enzima u kontrolnoj,eksperimentalnoj niti između dvije skupine. ZAKLJUČAK: Konzumacija kokošjih jaja nije utjecala na izražaj gena enzima kod sportaša.
Abstract (english)
mRNA expression of enzymes COX1, COX2, eNOS and iNOS in athletes consuming enriched hen eggs in n-3 polyunsaturated fatty acids, lutein, selenium and vitamin E STUDY AIM: This research aimed to examine the effects of consumption of chicken eggs enriched with n-3 PUFA, lutein, selenium and vitamin E on enzymes involved in the production of vasoactive metabolites (COX1, COX2, eNOS, iNOS). STRUCTURE OF THE STUDY: The study was organized as randomized, double-blind, and placebo-controlled. PARTICIPANTS AND METHODS: Controlled, interventional study (Clinical Trials ID: NCT04564690) was conducted in healthy young athletes (N = 14) who consumed 3 hen eggs per day for 3 weeks. Nutri4 group (N = 8) consumed enriched eggs (n-3 PUFAs:1026 mg/day, lutein:616 mg/day, selenium:0,0138 mg/day and vitamin E:1098 mg/day), and control subjects (N = 6) consumed ordinary eggs (n-3 PUFAs:438 mg/day, lutein:110 mg/day, selenium:0,02035 mg/day, vitamin E: 595 mg/day). Blood samples were collected before and after eggs consumption. mRNA of COX1, COX2, eNOS and iNOS enzymes expression in peripheral blood leukocyte was measured by qPCR method (BioRAD, CFX96 Real- Time System), normalized to GAPDH housekeeping gene. ANOVA was conducted for statistical analysis with Tukey's post hoc test; p<0.05 was considered statistically significant. RESULTS: The consumption of enriched chicken eggs did not cause a significant change in the gene expression of the enzyme in the control, experimental or between the two groups. CONCLUSION: Consumption of hen eggs did not affect gene expression of those enzymes in athletes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:707532
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2024-03-12 12:10:50