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master's thesis
Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora
2022. urn:nbn:hr:152:213579
Stepančević, Stefan
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Stepančević, S. (2022). Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:213579

Stepančević, Stefan. "Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:213579

Stepančević, Stefan. "Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:213579

Stepančević, S. (2022). 'Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:213579

Stepančević S. Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:213579

S. Stepančević, "Bijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:213579

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Metadata
TitleBijelim vinom potaknuta endotel-ovisna dilatacija kod Sprague-Dawley štakora
Title (english)White wine - induced endothelium-dependent vasorelaxation in Sprague-Dawley Rats
AuthorStefan Stepančević
MentorZrinka Mihaljević (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraţivanja: Ispitati vazodilatacijski potencijal i antioksidativni status bijelih vina proizvedenih različitim tehnološkim procesima. Nacrt studije: Eksperimentalna studija na izoliranim organima laboratorijskih ţivotinja. Ispitanici i metode: Kao uzorak za aortalne prstenove koristili smo torakalna aortu SpragueDawley štakora. Nakon izolacije, aortu smo odloţili u Petrijevu zdjelicu s oksigeniranom Krebs – Henseleitovom otopinom. Aortu smo potom smjestili u sustav tkivnih kupelji za izolirane organe te nakon sat vremena stabilizacije dodali noradrenalin i acetilkolin. Nakon ispiranja dodali smo uzorke bijelog vina u kumulativnoj dozi 0,1-8 ‰. Rezultati: Vazorelaksacija u grupama uzoraka bijelog vina WW1-3 i WW4-6 bila je znatno jača nego u grupama uzoraka bijelog vina WW 7-9 i WW 10-12. Predtretman aortnih prstenova s LNAME-om potpuno je poništio vazodilatatorni odgovor na sve uzorke bijelog vina. Sadrţaj fenola i flavonoida u grupama uzoraka WW 7-9 i WW 10-12 znatno je sniţen u usporedbi s grupama WW1-3 i WW4-6. FRAP i TBARS su bili znatno sniţeni u grupama WW7-9 i WW10-12 u odnosu na grupe WW1-3 i WW4-6, dok je DPPH 50 % EC bio znatno povišen. Zaključak: Rezultati pokazuju da je bijelim vinom potaknuta vazorelaksacija ovisna o NO i upućuju na mogućnost povećanja antioksidativnog kapaciteta i vazodilatacijskog potencijala bijelog vina korištenjem različitih postupaka proizvodnje, ovisno o sadrţaju kvercetina.
Abstract (english)
Objectives: The main goal of this study was to examine the vasodilatory potential and antioxidant status of white wines produced by different technological processes. Study Design: Experimental study on isolated organs of laboratory animals. Participants and methods: Sprague-Dawley rat thoracic aorta was used as a sample for aortic rings. After isolation, the aorta was placed in a Petri dish with oxygenated Krebs - Henseleit solution. The aorta was placed in a tissue bath system for isolated organs and noradrenaline and acetylcholine were added after one hour of stabilization. After rinsing, we added samples of white wine in cumulative doses 0.1-8 ‰. Results: Vasorelaxation in groups of wine samples WW1-3 and WW4-6 was much stronger than that of WW7-9 and WW10-12 groups. Pretreatment of the aortic rings with L-NAME completely abolished vasodilator response to all white wine samples. Phenol and flavonoid content in WW 7-9 and WW 10-12 groups of wine samples compared to WW1-3 and WW4-6 groups were significantly decreased. FRAP and TBARS were significantly decreased in WW7-9 and WW10-12 groups of wine samples compared to WW1-3 and WW4-6 groups, and DPPH 50 % EC was significantly increased. Conclusion: The results show that white wine induced vasorelaxation is NO-dependent and suggest the possibility of increasing the antioxidant capacity and vasodilatory potential of white wine using different production methods, depending on the quercetin content.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:213579
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-22 07:52:23