| Abstract | Cilj istraživanja: Cilj je studije ispitati postoji li povezanost ekspresije proteina za popravak DNA s različitim vrstama i karakteristikama kolorektalnog karcinoma. Materijali i metode: Analizirani su podatci 45 novodijagnosticiranih kolorektalnih karcinoma s imunohistokemijski ispitanom ekspresijom proteina za popravak DNA s Odjela za patologiju i citologiju OŽB Vinkovci, u razdoblju od lipnja 2015. godine do svibnja 2016. godine. Rezultati: Od 45 nalaza biopsija, 27 je muškaraca (60 %) i 18 žena (40 %). Prosječna je dob 67 (12) godina. Pacijenti su podijeljeni u dvije skupine s obzirom na status mikrosatelitske nestabilnosti, MSS (mikrosatelitski stabilni karcinomi) te MSI (mikrosatelitski nestabilni karcinomi). Grupi MSS pripada 37 (82 %), a grupi MSI osam (18 %) karcinoma. Uspoređujući navedene grupe nije utvrđena statistički značajna razlika: u prosječnoj dobi oboljelih (U = 114,5; p = 0,319), u učestalosti pojedinog spola (2 = 1,071; p = 0,301), u učestalosti određene patohistološke vrste (p = 0,139), u učestalosti metastatski pozitivnih limfnih čvorova (2 = 0,033; p = 0,862) te u učestalosti vaskularne invazije, perineuralne invazije i prodora u limfne žile (p > 0,05). Svih osam karcinoma MSI grupe lokalizirani su u proksimalnom kolonu što je statistički značajno češće u odnosu na MSS karcinome (p < 0,001). Prosječni promjer novotvorina MSI grupe iznosi 6,3 (2) cm, a novotvorina MSS grupe 5,1 (2,2) cm, no razlika nije statistički značajna (U = 96,5; p = 0,128). Zaključak: Učestalost od 18 % MSI karcinoma opravdava imunohistokemijsko ispitivanje enzima za popravak DNA kod svih pacijenata s kolorektalnim karcinomom. Osim toga, razlika u lokalizaciji MSI i MSS novotvorina ukazuje kako posebnu pozornost treba posvetiti detaljnijem pregledu proksimalnog kolona u bolesnika kod kojih se zbog anamnestičkih podataka sumnja na MSI karcinom. |
| Abstract (english) | Objective: The aim of the study is to examine whether there is a connection between expression of DNA repair proteins and different types and characteristics of colorectal cancer. Materials and Methods: Data of 45 newly diagnosed colorectal cancers were analysed by immunohistochemically examined expression of DNA repair proteins from the Department of Pathology and Cytology of the General County Hospital Vinkovci in the period from June 2015 to May 2016. RESULTS: Of the 45 biopsy results, 27 were men's (60%) and were 18 women's (40%). The average age was 67 (12). Patients were divided into two groups, according to the status of microsatellite instability, MSS (microsatellite stable cancers) and MSI (microsatellite unstable cancers). 37 cancers (82%) belong to group MSS, and 8 (18%) cancers belong to group MSI. When comparing the abovementioned groups, statistically significant difference was not determined when it comes to: the average age of patients (U = 114.5; p = 0.319), the frequency of a particular gender (2 = 1.071; p = 0.301), the frequency of a certain histopathological type (p = 0.139), the frequency of metastatic positive lymph nodes (2 = 0.033; p = 0.862), and the frequency of vascular invasion, perineural invasion and penetration into the lymphatic vessels (p > 0.05). All 8 cancers of MSI group are localised in the proximal colon that is statistically significantly more frequent in relation to MSS cancer (p < 0.001). The average diameter of neoplasms of MSI group is 6.3 (2) cm, and neoplasms of MSS group 5.1 (2.2) cm, but the difference was not statistically significant (U = 96.5; p = 0.128). CONCLUSION: The frequencyof 18% of MSI cancers justifies immunohistochemical testing of DNA repair enzymes in all patients with colorectal cancer. In addition, differences in localisation of MSI and MSS neoplasms suggests that particular attention should be paid to a more detailed examination of the proximal colon in patients who are suspected to suffer from MSI cancer due to the anamnesis. |
