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undergraduate thesis
Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi
2018. urn:nbn:hr:152:317799
Periš, Antonio
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Periš, A. (2018). Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:317799

Periš, Antonio. "Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:317799

Periš, Antonio. "Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:317799

Periš, A. (2018). 'Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:317799

Periš A. Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:317799

A. Periš, "Citotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:317799

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Metadata
TitleCitotoksičnost derivata kinazolinona na stanice koje rastu u 2D i 3D kulturi
Title (english)Cytotoxicity of kinazolinone derivatives on 2D and 3D cell cultures
AuthorAntonio Periš
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberTeuta Opačak-Bernardi (predsjednik povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Biochemistry
Abstract
Uvod: Dušikom bogati heterociklički spojevi, kinazolinoni,, su ubikvitarne farmakološke jedinice zastupljene u raznim bioaktivnim prirodnim i novosintetiziranim kemijskim spojevima. Stabilnost kinazolinonske jezgre te jednostavna sinteza strukturalno i funkcionalno različitih derivata utjecali su na njihovu važnost kao protutumorskih lijekova. Ciljevi istraživanja: Odrediti razlikuje li se citotoksični učinak novih derivata kinazolinona na normalnim i tumorskim stanicama u uvjetima 2D i 3D kulture in vitro. Materijali i metode: Citotoksičan učinak novih derivata kinazolinona praćen je na tri adherentne tumorske stanične linije (HeLa, CaCo-2 i NCI-H358) i jednoj normalnoj staničnoj liniji (MDCK1) u 2D kulturi te na staničnim linijama HeLa, CaCo-2 i NCI-H358 u 3D kulturi. Stanična vijabilnost određena je MTT testom nakon inkubacije od 72 h. Rezultati: Novi derivati kinazolinona djelovali su citotoksično pri koncentraciji od 1x10-4 M na 2D i 3D kulturu testiranih staničnih linija. Najbolji učinak postigao je derivat 3, a najosjetljivija je bila HeLa stanična linija. Zaključak: Razlikuje se citotoksični učinak novih derivata kinazolinona u uvjetima 2D i 3D kulture in vitro. Inhibicija rasta ovisi o koncentraciji spoja i vrsti stanične linije.
Abstract (english)
Introduction: Nitrogen-rich heterocyclic compounds, quinazolinones, are ubiquitous pharmacological units represented in various bioactive natural and newly synthesized chemical compounds. The stability of the quinazolinone core and simple synthesis of structurally and functionally diverse derivatives have influenced their importance as antineoplastic agents. Research objectives:. The aim was to determine whether there is a difference in the cytotoxic effect of novel quinazolinone derivatives on normal and tumour cell lines under 2D and 3D cell culture conditions in vitro. Materials and methods: The cytotoxic effect of novel quinazolinone derivatives was monitored on three adherent tumor cell lines (HeLa, CaCo-2 and NCI-H358) and one normal cell line (MDCK1) in 2D culture and on HeLa, CaCo-2 and NCI-H358 cell lines in 3D culture. The cell viability was determined by MTT assay after 72 hour incubation. Results: The novel quinazolinone derivatives acted cytotoxic at a concentration of 1x10-4 M on the 2D and 3D cultures of the tested cell lines. The best effect was achieved by the derivative 3, and the most sensitive was the HeLa cell line. Conclusion: There is a difference in cytotoxic effect of novel quinazolinone derivatives in the 2D and 3D cultures in vitro. The inhibition of growth depends on the concentration of the compound and the cell line type.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:317799
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
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Created on2018-12-13 12:37:24